Summary of results of genetic and reverse genetic analyses of Arabidopsis polyadenylation factor subunits

This table summarizes published results (from our labs and from other groups) of genetic and reverse genetic studies of plant polyadenylation factor subunits. It will be updated periodically, as new information becomes available.

factor or enzyme
Arabidopsis gene
mutant
over-expression
references
Poly(A) polymerase
 

At1g17980

essential (insertion mutations)

 

Meeks 2005 (Ph.D. thesis, University of Kentucky); Meeks et al., 2009
At2g25850
essential (insertion mutations)
  Meeks 2005 (Ph.D. thesis, University of Kentucky); Meeks et al., 2009
At3g06560

essential (insertion mutations);

selfed progeny of heterozygotes show suggestions of haplo-insuffiency

  Meeks 2005 (Ph.D. thesis, University of Kentucky); Meeks et al., 2009
At4g32850
essential (insertion mutations)
  Meeks 2005 (Ph.D. thesis, University of Kentucky); Meeks et al., 2009
CPSF
 
CPSF160
At5g51660
 
CPSF100
At5g23880
homozygotes are embryo-lethal (insertion mutation)
Tzafrir et al., 2004
CPSF73(I)

At1g61010

essential (insertion mutations)

overexpression using the 35S promoter is lethal;

over-expression with the "native" promoter results in male sterility

Xu et al., 2006
CPSF73(II)
At2g01730

essential (insertion mutations)

hemizygous plants are defective in female gametogenesis

  Xu et al., 2004
CPSF30
At1g30460

not essential (insertion);

insertion mutants are oxidative stress-tolerant, dwarfed

Delaney et al., 2006

Zhang et al., 2008

FY
At5g13480

essential (insertions);

some mutant alleles situated near the 3' end of the gene are viable, show a delay in flowering time

 

Simpson et al., 2003

Henderson et al., 2005

CstF
 
CstF77
At1g17760
mutants are affected in silencing of FLC gene expression
Liu et al., 2010
CstF64
At1g71800
mutants are affected in silencing of FLC gene expression
Liu et al., 2010
CstF50

At5g60940

essential (insertion mutation)
  Dampanaboina, L. 2011 (Ph.D. thesis, University of Kentucky)
Fip1
   

At5g58040

essential (insertion mutations)

 

Forbes et al., 2006
At3g66652
   
CFIm25
 

At4g29820

 

 
At4g25550
   
CFIm68        
 
At1g13190
     
 
At5g55670
     
Clp1p
 

At3g04680

essential (insertion mutation)

 

Xing et al., 2008
At5g39930
   
Pcf11
   

At2g36480

 

 
At5g43620
   
At1g66500
   
At4g04885
not essential (insertion); homozygous mutants have a delayed flowering phenotype
  Xing et al., 2008
PabN
 

At5g65260

 

 
At5g51120
   
 
At5g10350
     
Symplekin        
At5g01400
mutations affect RNA silencing, flowering (ESP4)
  Herr et al., 2006
At1g27590/At1g27595