Animal & Food Sciences

James C. Matthews, Ph.D.

Associate Professor
Beef Cattle Nutrition, Ruminant Nutritional Physiology, Molecular Biology, and Transport of Nutrients and Drugs
Department of Animal & Food Sciences
University of Kentucky
808 W. P. Garrigus Bldg.
Lexington, KY 40546-0215
Phone: (859) 257-7513
FAX: (859) 257-3412
E-mail: jmatthew@uky.edu

CURRENT STATUS

Associate Professor (January 2004) in Animal Sciences in the area of beef/ruminant nutrition at the University of Kentucky, Lexington, with an average 80:20% research:teaching appointment since 1998. My program of research in nutritional physiology has emphasized the molecular study and characterization of nutrient (and drug) transporters, and enzymes that either produce or metabolize transporter substrates. Because of their influence on nutrient assimilation and use, my program has emphasized the study of tissue-, age-, and/or diet-dependent expression and activity of these proteins. My research interests and efforts have become focused on identifying if and how the energy and/or nitrogen status of cells regulates/coordinates the expression and function of enzyme/transporter “functional units”. Accordingly, most of our research projects include multiple protein expression profiling. However, my broad research stratagem is not to study/characterize complete proteomes (or transcriptomes) but rather to understand how constitutive and inducible aspects of specific targeted biochemical pathways are regulated, in response to a given physiological state.

PRODUCTIVITY SUMMARY

Publications

Papers 23 Abstracts 35 Patents 1

Invited Book & Symposia Chapters 11 GenBank Deposits 7

Research Funding

Total    $1,786,312 Extramural $1,664,497 Intramural $121,815

Teaching

Course (credits)           Position, Responsibility      Semesters

ASC 689 (3) Instructor, 100% 5

ASC 683 (2) Instructor, 100% 3

ASC 680 (4)          Guest Instructor, 10% 4

GEN 300 (1-2)      Co-Instructor, 13% 3

ABT 395 (3-4) Coordinator, 100% 2

ABT/ASC/BIO 395 (2-4) Research Mentor, 100% 11

EDUCATION

Postdoctoral Research Fellow - University of Florida College of Medicine, Gainesville, FL. May 1995 to December 1997. Dual appointment in the Department of Biochemistry and Molecular Biology and the Department of Pediatrics.
Mentors: Michael S. Kilberg, Ph.D.; Donald A. Novak, M.D.

Ph.D. & M.S. - Virginia Tech, College of Agriculture and Life Sciences, Blacksburg, VA. September 1989 to May 1995. Animal Science (Ruminant Nutrition).
Advisor: Kenneth E. Webb, Jr., Ph.D.

B.S. - Rutgers University, Cook College, New Brunswick, NJ. September 1985 to December 1988. Animal Science (Preparation for Research)/Nutrition.
Research mentor: James E. Wohlt.

TEACHING PROGRAM (Current)

Physiology of Digestion and Nutrient Absorption (ASC 689, 3 credits) - Instructor (100%, 48 lecture periods), taught fall 1998, and spring 2000, 2001, 2002, 2004. Prerequisite: BCH 601 or equivalent. This course was developed in fall 1998. Course name (formerly Principles of Animal Nutrition) and major curriculum changes were approved spring 2000. A request to increase the series level (from 580 to 689) to reflect the rigor of the course content was approved fall 2002. I will continue to teach this course the spring of even-numbered years.

Protein Metabolism (ASC 683, 2 credits) - Instructor (100%, 32 lecture periods), taught fall 1999, 2001, 2003. Prerequisite BCH 601 or equivalent. I developed this course in the fall of 1999. I will continue to teach this course the fall of odd-numbered years.

Independent Study in Biotechnology (ABT 395, 3-4 credits) - Instructor (100%), Responsible for coordinating the written and oral presentations of student (10-20, typically) research projects (began fall 2004).

ADVISING PROGRAM

Advisee Dissertations and Theses

Brian M. Zanghi. 2005. Characterization and Regulation of In Vivo and In Vitro Functional Activities of Canine PepT1. Ph.D. Dissertation, University of Kentucky, Lexington. Advisor: J. C. Matthews.

Geri L. Sipe. 2004. Expression of High-affinity Glutamate Transporters and Glutamine Synthetase by Liver, Longissimus Dorsi and/or Adipose Tissues of Finishing Cattle Is Differentially Altered by Chlortetracycline and Synovex-S. Advisor: J. C. Matthews.

Sonya J. Gissendanner. Expression of Eaac1 and GLT-1 High-affinity Glutamate Transporters by Liver, Kidney, Intestinal Epithelia, Longissimus Dorsi Muscle and Adipose Tissues of Developing Angus Cattle. 2004. M.S. Thesis, University of Kentucky, Lexington. Advisor: J. C. Matthews.

Chad A. Woods. Substrate Recognition and Regulation of Canine PepT1 Function Using a MDCK Model. 2001. M.S. Thesis, University of Kentucky, Lexington. Advisor: J. C. Matthews.

Kendall C. Swanson. Mechanisms Regulating Pancreatic a-Amylase Expression and Secretion in Ruminants. 2000. Ph.D. Dissertation, University of Kentucky, Lexington. Advisor: D. L. Harmon, Committee member: J. C. Matthews.

Julie A. Howell. Tissue Distribution of Na⁺-Dependent Glutamate Transporters in Sheep and Cattle and Differential Tissue Expression of GLT-1 and EAAC1 in Growing Versus Non-Growing Lambs. 2000. M.S. Thesis, University of Kentucky, Lexington. Advisor: J. C. Matthews.

Current Undergraduate, Graduate and Postdoctoral Student Advising

Undergraduate
Four Agricultural Biotechnology and one Animal Science students.

M.S.
Edwina Miles, M.S. (started August 2003; ongoing 2^nd year).

Ph.D.
Louis Dionissopoulus, Ph.D. (started January 2004; ongoing 2^nd year).

Postdoctoral Fellow
Brian Zanghi (January 2005 - present, co-advisor: J. A. Boling)
Shengfa Liao (April 2005 - present).

RECENT PUBLICATIONS

Refereed Articles (of 23)

Benson, J.A., K.C. Swanson, J.C. Matthews, and D.L. Harmon. Influence of Starch Hydrolysate and/or Casein on Pancreatic Exocrine Secretion and Plasma Hormone Concentrations in Beef Steers. Journal of Animal Science 82:1781-1787. 2004.

Novak, D., F. Quiggle, and J. C. Matthews. 2004. Androgen Secretion by Rcho-1 Cells is Independent of Extracellular Glutamate Concentration. Placenta 25:548-552.

Fan, M. Z., J. C. Matthews, N. M. P. Etienne, D. G. Burrin, and B. Stoll. 2004. Expression of Apical Membrane L-glutamate Transporters in Neonatal Porcine Epithelial Cells Along the Small Intestinal Crypt-villus Axis. American Journal of Physiology- Gastrointestinal and Liver Physiology 287:G385-G398.

Swanson, K. C., J. A. Benson, J. C. Matthews, and D. L. Harmon. 2004. Influence of Starch Hydrolysate and/or Casein on Pancreatic Exocrine Secretion and Plasma Hormone Concentrations in Beef Steers. Journal of Animal Science 82:1781-1787.

Rodriguez, S. M., K. C. Guimaraes, J. C. Matthews, K. R. McLeod, R. L. Baldwin, VI and D. L. Harmon. 2004. Influence of Abomasal Carbohydrates on Small Intestinal Sodium-dependent Glucose Cotransporter Activity and Abundance in Steers. Journal of Animal Science 82:3015-3023.

Swanson, K. C., J. C. Matthews, C. A. Woods, and D. L. Harmon. 2003. Influence of Substrate and/or Neurohormonal Mimic on In Vitro Pancreatic Enzyme Release from Calves Postruminally Infused with Partially Hydrolyzed Starch and/or Casein. Journal of Animal Science 81:1323-1331.

Novak, D. A. and J. C. Matthews. 2003. Glutamate transport by Rcho-1 cells derived from rat placenta. Pediatric Research 53(5):1-5.

Howell, J. A., A. D. Matthews, T. C. Welbourne, and J. C. Matthews. 2003. Content of Ileal EAAC1 and Hepatic GLT-1 High-Affinity Glutamate Transporters is Increased in Growing Versus Non-Growing Lambs, Paralleling Increased Tissue Concentrations of D- and L-Glutamate and Plasma Glutamine and Alanine. Journal of Animal Science 81:1030-1039.

Swanson, K. C., J. C. Matthews, C. A. Woods, and D. L. Harmon. 2002. Post-Ruminal Administration of Partially Hydrolyzed Starch and Casein Influences Pancreatic a-Amylase Expression in Calves. Journal of Nutrition 132:376-381.

Matthews, J. C. and K. J. Anderson. 2002. Recent Advances in Amino Acid Transporters and Excitatory Amino Acid Receptors. Current Opinion in Clinical Nutrition and Metabolic Care 5:77-84.

Howell, J. A., A. D. Matthews, K. C. Swanson, D. L. Harmon, and J. C. Matthews. 2001. Molecular Identification of High-Affinity Glutamate Transporters in Sheep and Cattle Forestomach, Intestine, Liver, Kidney, and Pancreas. Journal of Animal Science 79:1329-1336.

Swanson, K. C., J. C. Matthews, A. D. Wilson, J. A. Howell, C. J. Richards, and D. L. Harmon. 2000. Influence of Dietary Carbohydrate Source and Energy Intake on Pancreatic a-Amylase Expression in Lambs. Journal of Nutrition 130:2157-2165.

Welbourne, T., and J. C. Matthews. 1999. Glutamate Transport and Renal Function. American Journal of Physiology 277(Renal Physiol. 46):F501-F505.

Matthews, J. C., M. J. Beveridge, E. Dialynas, A. Bartke, M. S. Kilberg, and D. A. Novak. 1999. Placental Cationic and Anionic Amino Acid Transporter Expression in Growth Hormone Overexpressing, Null IGF-II or Null IGF-1 Receptor Mice. Placenta 20:639-650.

Matthews, J. C., M. J. Beveridge, M. S. Malandro, J. D. Rothstein, M. Campbell-Thompson, J. E. Verlander, M. S. Kilberg, and D. A. Novak. 1998. Activity and Protein Localization of Multiple Glutamate Transporters in Gestation Day 14 Versus Day 20 Rat Placenta. American Journal of Physiology 274 (Cell Physiol. 43):C603-614.

Recent Invited Book Chapters (of 8)

Zanghi, B. M., and J. C. Matthews. 2005. Physiological Importance and Mechanisms of Protein Hydrolysate Absorption. In: V. Pasulouppi (Ed.) Protein Hydrolysates in Nutrition and Biotechnology. Chapter V, pp. 1-43. Kluwar Academic Publishers, Amsterdam. Accepted.

Matthews, J.C. and G.L. Sipe. Patterns and Putative Regulatory Mechanisms of High-Affinity Glutamate Transporter Expression by Ruminants. pp. 263-287. In: Proceedings of the Xth International Symposium on Ruminant Physiology, Copenhagen, Denmark. 2005.

Matthews, J. C.. Expression and Function of Non-Organelle Glutamate Transporters To Support Peripheral Tissue Function. 2005. In: S. Gill and O. Pulido (Eds.) Glutamate Receptors in Peripheral Tissues: Excitatory Transmission Outside the CNS. Chapter 3, pp. 47-75. Kluwer Academc/Plenum Press, New York.

Krehbeil, C. R., and J. C. Matthews. Absorption of Amino Acids and Peptides. 2003. In: J. P. F. D’Mello (Ed.) Amino Acids in Animal Nutrition, 2^nd edition. Chapter 3, 41-70. CAB International, Wallingford.

Matthews, J. C.. 2000. Mechanisms of Peptide and Amino Acid Transport. In: J. P. F. D’Mello (Ed.) Farm Animal Metabolism and Nutrition: Critical Reviews. Chapter 1, 3-23. CAB International, Wallingford.

Recent Invited Seminars (of 12)

Patterns and Putative Regulatory Pathways of High-Affinity Glutamate Transporter Expression by Ruminants. January 15, April 2005. Virginia Tech Department of Dairy Science Seminar, Blacksburg, VA.

Nutritional Regulation of Gene Expression: Regulation of High-Affinity Glutamate Transporter Expression in Developing Ruminants. August 30 to September 4, 2004. Invited Main Paper for Nutrient Regulation of Gene Expression, X^th International Symposium on Ruminant Physiology, Copenhagen, Denmark.

Coordinated Expression of Proteins that Transport and Metabolize Glutamate to Support Altered Gut and Peripheral Tissue Function: A work in progress. April 27, 2004. Virginia Tech Nutrition Seminar, Blacksburg, VA.

Altered Expression of High-Affinity Glutamate Transporters to Support Changing Gut and Peripheral Tissue Metabolism of Developing Ruminants. February 17, 2004. Texas A&M Faculty of Nutrition Seminar, College Station, TX.

Patent

Compositions and Methods for Increasing Amino Acid Absorption in Mammals (U.S. Patent number 6803186). G. M. Davenport (The IAMS Company) and J. C. Matthews (PI; University of Kentucky). Awarded October 12, 2004.

Recent Abstracts (of 35)

Sipe, G. L., B. M. Zanghi, G. Wu, J. A. Boling, and J. C. Matthews. 2005. Blood Chemical and Plasma Amino Acid Profiles of Old Versus Mature Young Beef Cows. Accepted by Journal of Animal Science 83 (suppl. 1): abstract no. 10163).

Zanghi, B. M., N. M. P. Etienne, A.D. Matthews, E. D. Miles, G. M. Davenport, and J. C. Matthews. 2005. Cloning and In Vitro Characterization of Dog PepT1 and Development of a Polarized Cell Model to Study PepT1 Trafficking and Regulation. In press, Journal of Animal Science 83 (suppl. 1): abstract no. 9930.

Zanghi, B. M., G. L. Sipe, G. M. Davenport, and J. C. Matthews. 2005. Feeding of Chicken or Soy Protein-Based Diet Differentially Affects In Vivo PepT1 Uptake Capacity in Dogs. In press, Journal of Animal Science 82 (suppl. 1): abstract no. ).

Miles, E. D., J. A. Boling, and J. C. Matthews. 2005. Specific Glutamate and Nucleoside Transport Activities of Madin-Darby Bovine Kideny (MDBK) Cells Are Inhibited by the Ergopeptide Bromocryptine. In press, Journal of Animal Science 83 (suppl. 1): abstract no. 10032).

Kitts, S. E., J. C. Matthews, G. L. Sipes, T. S. Rumsey, T. H. Elasser, R. L. Baldwin, and K. R. McLeod. 2004. Effects of Chlortetracycline (CTC) and Steroidal Implant on Growth and Plasma Growth Hormone (GH) and Thyroid-Stimulating Hormone (TSH) After Challenge with Thyroid-Releasing Hormone (TRH) and GH-Releasing Hormone (GHR) on Finishing Beef Steers. Journal of Animal Science 82 (suppl. 1):41.

Sipe, G. L., S. E. Kitts, K. R. McLeod, and J. C. Matthews. 2004. Chlortetracycline, but not Synovex-S, Differentially Affects Tissue Expression of High-Affinity Glutamate Transporters in Fattening Steers. Journal of Animal Science 82 (suppl. 1):42.

Zanghi, B. M., G. L. Sipe, G. M. Davenport, and J. C. Matthews. 2004. Evaluation of Glycylsarcosine and Cefadroxil as Substrates for Non-invasive Determination of Canine Small Intestine PepT1 Capacity and Demonstration That Maximal Cefadroxil Absorption Occurs When Consumed 4 h After Meal Ingestion. Journal of Animal Science 82 (suppl. 1): 245.

Gissendanner, S. J., N. M. P. Etienne, and J. C. Matthews. 2003. Differential Expression of EAAC1 and GLT-1 Glutamate Transporters by Bovine Epithelial Tissues is not Altered by Physiological Development. FASEB Journal 17(5):A305.

Gissendanner, S. J., N. M. P. Etienne, K. R. McLeod, and J. C. Matthews. 2003. The Pattern of EAAC1 and GLT-1 Glutamate Transporter Expression by Skeletal Muscle and Adipose Tissues of Fattening Cattle Differs from that of Glutamine Synthetase. FASEB Journal 17(5):A738.

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