Laboratory Resources

Page Index
Reproductive Physiology	Nutrient Transport	Equine Nutrition
Lactation Physiology	Poultry Nutrition	Food Microbiology
Dairy Chemistry	Swine Nutrition	Food Protein Lab
Food Chemistry	Ruminant Nutrition

Reproductive Physiology Lab

Major Equipment:

Liquid Scintillation Counter

High capacity, low speed refrigerated centrifuge

Automated' pipetting stations

Dubnoff metabolic shaking incubators

Assorted equipment for agarose and polyacrylamide gel

Electrophoresis and blotting procedures

Procedures:

Radioimmunoassay

Short term tissue culture

Northern and Western blotting

Location of Lab: 419 WPG
Contact Person(s):
Bill Silvia 7-7541
Susan Hayes 7-4168

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Lactation Physiology Lab

Major Equipment:

AutoAnalyzer II

Microplate Reader & computer interface

Microplate washer

Research Microscope

- bright field, dark field, phase contrast
- fluorescence attachment
- photomicrography

Refrigerated high-speed centrifuge

Cytospin centrifuge

Routine Procedures:

Blood urea nitrogen

Rumen ammonia

ELISA assays (antibody titers)

Milk microbiological cultures

Liver Cu, Zn, Fe

Plasma Cu, Zn, Fe

Plasma ceruloplasmin

Plasma antioxidant activity

Intramammary experimental challenge models

Phagocytosis & bactericidal assays for blood neutrophils

Location of Lab: 421 WPG
Contact Person(s):
Bob Harmon; 7-7545
Bernice Smith; 7-7546

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Nutrient Transport Lab

Major equipment:

HTS 7000 spectrophotometer (6- to 392-well microtiter plate reader; UV, absorbance, fluoresence reading capabilities).

CO2 incubators

Cell culture hood

Hybridization oven

UV crosslinker

Gel electrophoresis units (horizontal/agarose, and vertical/PAGE)

Superspeed centrifuge (1.5, 10, 50, 250, 500 mL tube or bottle rotors)

Routine procedures:

RNA (A260, torulla yeast RNA std) quantification

DNA (A260, salmon sperm DNA) quantification

Protein (A590, bovine serum albumin; Lowry) quantification

Reverse transcriptase

PCR

TA cloning

Enzymatic restriction of cDNA

Northern and immunoblot (Western)

Primary and immortalized cell culture

Whole-cell transport

Location of Lab: 821A WPG
Contact Person(s):
Jamie Matthews 7-7513

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Poultry Nutrition Lab

Major Equipment:

Bomb calorimeter

Filter fluorometer

Routine procedures:

Selenium assay

Location of Lab: 621 WPG
Contact Person(s):
Austin Cantor 7-7531

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Equine Nutrition Laboratory

Major Equipment:

YSI Glucose/Lactate Analyzer

Refrigerated Centrifuge

-80 Freezer

Brinkman Homogenizer

UV-Visible Spectrophotometer

Scanning Thermocouple

Portable Large Animal Scale (2000 lb capacity)

Large Animal Treadmill (at Farm)

Portable Radiograph

Equine Heart Rate Monitor

Water bath, balances, etc

Routine Procedures:

Blood/plasma/serum chemistries (glucose, lactate, free fatty acids, triglycerides, glycerol)

Total protein

Plasma volume

Serum CK and AST

Radiographic bone density

Location of Lab: 616 WPG
Contact Person(s):
L. Lawrence 7-7509; Room 905
S. Hayes 7-4168; Room 419

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Food Microbiology Lab

Major Equipment:

General microbiological equipment

Autoclaves

Incubators

Water Baths

PCR Thermal Cycler

Laminar Flow Hood

Routine Procedures:

Microbial Enumeration

Microbial Identification

Microbial Challenge studies

Antibiotic Resistance studies

Fermentation studies

Location of Lab: 221 WPG
Contact Person(s):
Melissa Newman 7-5881

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Food Protein Lab

Dairy Chemistry Lab

Major Equipment:

Babcock test equipment

Some membrane equipment

Spray drying equipment

Instron

Routine Procedures:

Babcock fat test

Location of Lab: 216C & D WPG
Contact person(s):
C. Hicks 7-7538

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Swine Nutrition Lab

Major Equipment:

Bomb calorimeter (Parr)

Nanopure water purifier

Microwave digestion (2 units)

ELISA plate reader

Tecator Soxtec system reflux fat extraction unit

Instron machine

Location of Lab: 618/621 WPG
Contact person(s):
Dr. Noel Inocencio 7-7561

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Food Chemistry Lab

Major Equipment:

Gas Chromatograph with FID

GC/Mass Spectrometer

Microbalance

Large volume centrifuge (8,000 x g)

HPLC with diode array detector

UV/VIS spectrophotometer

Routine Procedures:

Flavor chemistry procedures

Contact Person(s):
Luke Boatright 7-5988

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Ruminant Nutrition Lab

Major Equipment:

UV/VIS spectrophotometer

COBAS centrifugal analyzer

Atomic absorption spectrophotometer (includes graphite furnace)

HPLC with UV/VIS and fluorescent detection

LECO N-analyzer

Gas chromatograph with FID

Standard and Ankom fiber analysis equipment

Routine Procedures:

Metabolites

Ruminal Volatile Fatty Acid (VFA)

Blood VFA

D- And L-Lactate (Enzymatic)

Lactic Acid (Barker Summerson)

Pyruvate Analysis

Acetoacetate Analysis

b-Hydroxybutyrate Analysis

L-Alanine Analysis

Glutamate Analysis

Glutamine Analysis

Free Fatty Acid Analysis

Vitamin E In Plasma

Blood Glucose Specific Activity

Enzymes

Amylase

Trypsin

Chymotrypsin

Carboxypeptidase A

Ruminal Fluid Urease Assay

Nitrogen

Kjeldahl Nitrogen And Protein

Acid Detergent Insoluble Nitrogen

Pepsin Insoluble Nitrogen

Protein Assay Using Bca (Pierce, No. 23225)

Ammonia

Fiber

Total Dietary Fiber In Foods

Neutral Detergent Fiber (Cell Wall Constituents)

Alternative Procedure For High Starch Samples

Acid Detergent Fiber (ADF)

Acid Detergent Lignin (ADL)

Filtrations Using Filter Paper For (ADF and NDF )

Crucible Cleaning Procedure

Ankom Method For ADF

Ankom Method For NDF

Ankom Method For Crude Fiber

Ankom Method For ADL Using Beakers

Markers

Acid Insoluble Ash

Atomic Absorption Analysis Of Chromic Oxide

Analysis Of Chromic Oxide By Wet Ashing

Chromic Oxide-Dry Ashing And Colorimetric

DTPA Extraction For Rare-Earth Element Analysis

Preparation Of Chromium And Cobalt EDTA

Indigestible Adf (IADF)

Other Analyses

Starch Analysis

Total Long Chain Fatty Acid Analysis

Analysis Of Total Lipid Content

Inorganic Phosphate Determination

Total Calcium Analysis

Brush Border Membrane Vesicles

Maltase Assay For Transport

Alkaline Phosphatase Assay For Transport

Microbial Analyses

Separation Of Bacteria And Protozoa

Bacterial Isolation From Rumen Contents

Determination Of D-Alanine

Purine Content Of Ruminal Microbes And Digesta

Tilley-Terry Two Stage In Vitro Fermentation

Van Soest Modification Of Tilley-Terry Procedure

Ankom In Vitro True Digestibility

Cobas Procedures

Measuring Absorbance At 280 Nm

Alpha Amino Nitrogen

Alpha Amino Nitrogen (Hydrolysates)

Plasma Amylase

Ammonia Nitrogen Using Gdh For Plasma

Ammonia Nitrogen Using Salicylate

Bca Soluble Protein

b-Hydroxybutyrate In Blood

Cholesterol In Plasma

Creatinine For Plasma And Urine

Chymotrypsin In Pancreatic Juice

Plasma Glucose Using Hexokinase

Inorganic Phosphorus

Free Fatty Acids In Plasma

Pah Analysis

Trypsin In Bovine Pancreatic Juice

Contact Person(s):
David L. Harmon 7-7516
Dani True 7-2977

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