Laboratory Resources

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Page Index
Reproductive Physiology	Nutrient Transport	Equine Nutrition
Mammalian Reproduction	Poultry Nutrition	Food Microbiology
Dairy Chemistry	Swine Nutrition	Food Protein Lab
Food Chemistry	Ruminant Nutrition

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Reproductive Physiology Lab

Major Equipment:

• Liquid Scintillation Counter
• High capacity, low speed refrigerated centrifuge
• Automated' pipetting stations
• Dubnoff metabolic shaking incubators
• Assorted equipment for agarose and polyacrylamide gel
• Electrophoresis and blotting procedures

Procedures:

• Radioimmunoassay
• Short term tissue culture
• Northern and Western blotting

Location of Lab: 419 WPG
Contact Person(s):
     Bill Silvia 7-7541
     Susan Hayes 7-4168

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Mammalian Reproduction Lab

Major Equipment:

• Research microscope
• Water-jacketed incubator
• Shaking incubator
• Thermal cycler
• Imaging system
• Spectrophotometer
• Refrigerated centrifuge

Routine Procedures:

• Genomic DNA isolation
• Protein isolation
• Total RNA isolation, purification and quantification
• Reverse transcription and PCR

Location of Lab: 218B WPG
Contact Person(s):
     Phillip Bridges; 7-4877

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Nutrient Transport Lab

Major equipment:

• HTS 7000 spectrophotometer (6- to 392-well microtiter plate reader; UV, absorbance, fluoresence reading capabilities).
• CO2 incubators
• Cell culture hood
• Hybridization oven
• UV crosslinker
• Gel electrophoresis units (horizontal/agarose, and vertical/PAGE)
• Superspeed centrifuge (1.5, 10, 50, 250, 500 mL tube or bottle rotors)

Routine procedures:

• RNA (A260, torulla yeast RNA std) quantification
• DNA (A260, salmon sperm DNA) quantification
• Protein (A590, bovine serum albumin; Lowry) quantification
• Reverse transcriptase
• PCR
• TA cloning
• Enzymatic restriction of cDNA
• Northern and immunoblot (Western)
• Primary and immortalized cell culture
• Whole-cell transport

Location of Lab: 821A WPG
Contact Person(s):
Jamie Matthews 7-7513

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Poultry Nutrition Lab

Major Equipment:

• Bomb calorimeter
• Filter fluorometer

Routine procedures:

• Selenium assay

Location of Lab: 621 WPG
Contact Person(s):
Austin Cantor 7-7531

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Equine Nutrition Laboratory

Major Equipment:

• YSI Glucose/Lactate Analyzer
• Refrigerated Centrifuge
• -80 Freezer
• Brinkman Homogenizer
• UV-Visible Spectrophotometer
• Scanning Thermocouple
• Portable Large Animal Scale (2000 lb capacity)
• Large Animal Treadmill (at Farm)
• Portable Radiograph
• Equine Heart Rate Monitor
• Water bath, balances, etc

Routine Procedures:

• Blood/plasma/serum chemistries (glucose, lactate, free fatty acids, triglycerides, glycerol)
• Total protein
• Plasma volume
• Serum CK and AST
• Radiographic bone density

Location of Lab: 616 WPG
Contact Person(s):
L. Lawrence 7-7509; Room 905
S. Hayes 7-4168; Room 419

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Food Microbiology Lab

Major Equipment:

• General microbiological equipment
• Autoclaves
• Incubators
• Water Baths
• PCR Thermal Cycler
• Laminar Flow Hood

Routine Procedures:

• Microbial Enumeration
• Microbial Identification
• Microbial Challenge studies
• Antibiotic Resistance studies
• Fermentation studies

Location of Lab: 221 WPG
Contact Person(s):
Melissa Newman 7-5881

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Food Protein Lab

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Dairy Chemistry Lab

Major Equipment:

• Babcock test equipment
• Some membrane equipment
• Spray drying equipment
• Instron

Routine Procedures:

• Babcock fat test

Location of Lab: 216C & D WPG
Contact person(s):
C. Hicks 7-7538

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Swine Nutrition Lab

Major Equipment:

• Bomb calorimeter (Parr)
• Nanopure water purifier
• Microwave digestion (2 units)
• ELISA plate reader
• Tecator Soxtec system reflux fat extraction unit
• Instron machine

Location of Lab: 618/621 WPG
Contact person(s):
Dr. Noel Inocencio 7-7561

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Food Chemistry Lab

Major Equipment:

• Gas Chromatograph with FID
• GC/Mass Spectrometer
• Microbalance
• Large volume centrifuge (8,000 x g)
• HPLC with diode array detector
• UV/VIS spectrophotometer

Routine Procedures:

• Flavor chemistry procedures

Contact Person(s):
Luke Boatright 7-5988

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Ruminant Nutrition Lab

Major Equipment:

• UV/VIS spectrophotometer
• COBAS centrifugal analyzer
• Atomic absorption spectrophotometer (includes graphite furnace)
• HPLC with UV/VIS and fluorescent detection
• LECO N-analyzer
• Gas chromatograph with FID
• Standard and Ankom fiber analysis equipment

Routine Procedures:

Metabolites

• Ruminal Volatile Fatty Acid (VFA)
• Blood VFA
• D- And L-Lactate (Enzymatic)
• Lactic Acid (Barker Summerson)
• Pyruvate Analysis
• Acetoacetate Analysis
• b-Hydroxybutyrate Analysis
• L-Alanine Analysis
• Glutamate Analysis
• Glutamine Analysis
• Free Fatty Acid Analysis
• Vitamin E In Plasma
• Blood Glucose Specific Activity

Enzymes

• Amylase
• Trypsin
• Chymotrypsin
• Carboxypeptidase A
• Ruminal Fluid Urease Assay

Nitrogen

• Kjeldahl Nitrogen And Protein
• Acid Detergent Insoluble Nitrogen
• Pepsin Insoluble Nitrogen
• Protein Assay Using Bca (Pierce, No. 23225)
• Ammonia

Fiber

• Total Dietary Fiber In Foods
• Neutral Detergent Fiber (Cell Wall Constituents)
• Alternative Procedure For High Starch Samples
• Acid Detergent Fiber (ADF)
• Acid Detergent Lignin (ADL)
• Filtrations Using Filter Paper For (ADF and NDF )
• Crucible Cleaning Procedure
• Ankom Method For ADF
• Ankom Method For NDF
• Ankom Method For Crude Fiber
• Ankom Method For ADL Using Beakers

Markers

• Acid Insoluble Ash
• Atomic Absorption Analysis Of Chromic Oxide
• Analysis Of Chromic Oxide By Wet Ashing
• Chromic Oxide-Dry Ashing And Colorimetric
• DTPA Extraction For Rare-Earth Element Analysis
• Preparation Of Chromium And Cobalt EDTA
• Indigestible Adf (IADF)

Other Analyses

• Starch Analysis
• Total Long Chain Fatty Acid Analysis
• Analysis Of Total Lipid Content
• Inorganic Phosphate Determination
• Total Calcium Analysis
• Brush Border Membrane Vesicles
• Maltase Assay For Transport
• Alkaline Phosphatase Assay For Transport

Microbial Analyses

• Separation Of Bacteria And Protozoa
• Bacterial Isolation From Rumen Contents
• Determination Of D-Alanine
• Purine Content Of Ruminal Microbes And Digesta
• Tilley-Terry Two Stage In Vitro Fermentation
• Van Soest Modification Of Tilley-Terry Procedure
• Ankom In Vitro True Digestibility

Cobas Procedures

• Measuring Absorbance At 280 Nm
• Alpha Amino Nitrogen
• Alpha Amino Nitrogen (Hydrolysates)
• Plasma Amylase
• Ammonia Nitrogen Using Gdh For Plasma
• Ammonia Nitrogen Using Salicylate
• Bca Soluble Protein
• b-Hydroxybutyrate In Blood
• Cholesterol In Plasma
• Creatinine For Plasma And Urine
• Chymotrypsin In Pancreatic Juice
• Plasma Glucose Using Hexokinase
• Inorganic Phosphorus
• Free Fatty Acids In Plasma
• Pah Analysis
• Trypsin In Bovine Pancreatic Juice

Contact Person(s):
David L. Harmon 7-7516
Dani True 7-2977

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