University of Kentucky College of Agriculture
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Laboratory Resources


Page Index

Reproductive Physiology Nutrient Transport Equine Nutrition
Mammalian Reproduction Poultry Nutrition Food Microbiology
Dairy Chemistry Swine Nutrition Food Protein Lab
Food Chemistry Ruminant Nutrition  

Reproductive Physiology Lab

Major Equipment:

  • Liquid Scintillation Counter
  • High capacity, low speed refrigerated centrifuge
  • Automated' pipetting stations
  • Dubnoff metabolic shaking incubators
  • Assorted equipment for agarose and polyacrylamide gel
  • Electrophoresis and blotting procedures

Procedures:

  • Radioimmunoassay
  • Short term tissue culture
  • Northern and Western blotting

Location of Lab: 419 WPG
Contact Person(s):
     Bill Silvia 7-7541
     Susan Hayes 7-4168

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Mammalian Reproduction Lab

Major Equipment:

  • Research microscope
  • Water-jacketed incubator
  • Shaking incubator
  • Thermal cycler
  • Imaging system
  • Spectrophotometer
  • Refrigerated centrifuge

Routine Procedures:

  • Genomic DNA isolation
  • Protein isolation
  • Total RNA isolation, purification and quantification
  • Reverse transcription and PCR

Location of Lab: 218B WPG
Contact Person(s):
     Phillip Bridges; 7-4877

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Nutrient Transport Lab

Major equipment:

  • HTS 7000 spectrophotometer (6- to 392-well microtiter plate reader; UV, absorbance, fluoresence reading capabilities).
  • CO2 incubators
  • Cell culture hood
  • Hybridization oven
  • UV crosslinker
  • Gel electrophoresis units (horizontal/agarose, and vertical/PAGE)
  • Superspeed centrifuge (1.5, 10, 50, 250, 500 mL tube or bottle rotors)

Routine procedures:

  • RNA (A260, torulla yeast RNA std) quantification
  • DNA (A260, salmon sperm DNA) quantification
  • Protein (A590, bovine serum albumin; Lowry) quantification
  • Reverse transcriptase
  • PCR
  • TA cloning
  • Enzymatic restriction of cDNA
  • Northern and immunoblot (Western)
  • Primary and immortalized cell culture
  • Whole-cell transport

Location of Lab: 821A WPG
Contact Person(s):
Jamie Matthews 7-7513

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Poultry Nutrition Lab

Major Equipment:

  • Bomb calorimeter
  • Filter fluorometer

Routine procedures:

  • Selenium assay

Location of Lab: 621 WPG
Contact Person(s):
Austin Cantor 7-7531

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Equine Nutrition Laboratory

Major Equipment:

  • YSI Glucose/Lactate Analyzer
  • Refrigerated Centrifuge
  • -80 Freezer
  • Brinkman Homogenizer
  • UV-Visible Spectrophotometer
  • Scanning Thermocouple
  • Portable Large Animal Scale (2000 lb capacity)
  • Large Animal Treadmill (at Farm)
  • Portable Radiograph
  • Equine Heart Rate Monitor
  • Water bath, balances, etc

Routine Procedures:

  • Blood/plasma/serum chemistries (glucose, lactate, free fatty acids, triglycerides, glycerol)
  • Total protein
  • Plasma volume
  • Serum CK and AST
  • Radiographic bone density

Location of Lab: 616 WPG
Contact Person(s):
L. Lawrence 7-7509; Room 905
S. Hayes 7-4168; Room 419

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Food Microbiology Lab

Major Equipment:

  • General microbiological equipment
  • Autoclaves
  • Incubators
  • Water Baths
  • PCR Thermal Cycler
  • Laminar Flow Hood

Routine Procedures:

  • Microbial Enumeration
  • Microbial Identification
  • Microbial Challenge studies
  • Antibiotic Resistance studies
  • Fermentation studies

Location of Lab: 221 WPG
Contact Person(s):
Melissa Newman 7-5881

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Food Protein Lab

Major Equipment

  • Bohlin Rheometer
  • Differential Scanning Calorimeter
  • Fluoromax Flourometer
  • Brinkman Polytron
  • Turner Fluorometer
  • LKB Laser Densitometer
  • Perkin Elmer Spectrophotometer
  • Biorad SDS-Page
  • Circulating Waterbath
  • Instron 4301

Routine Procedures

  • Protein Isolation/Purification
  • SDS-Page
  • DSC
  • Sensory Panels
  • Gel Strength
  • Dynamic Rheological Properties
  • Water Holding Capacity
  • Spectrophotometric Analysis
  • Protein Biochemistry Analysis

Dairy Chemistry Lab

Major Equipment:

  • Babcock test equipment
  • Some membrane equipment
  • Spray drying equipment
  • Instron

Routine Procedures:

  • Babcock fat test

Location of Lab: 216C & D WPG
Contact person(s):
C. Hicks 7-7538

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Swine Nutrition Lab

Major Equipment:

  • Bomb calorimeter (Parr)
  • Nanopure water purifier
  • Microwave digestion (2 units)
  • ELISA plate reader
  • Tecator Soxtec system reflux fat extraction unit
  • Instron machine

Location of Lab: 618/621 WPG
Contact person(s):
Dr. Noel Inocencio 7-7561

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Food Chemistry Lab

Major Equipment:

  • Gas Chromatograph with FID
  • GC/Mass Spectrometer
  • Microbalance
  • Large volume centrifuge (8,000 x g)
  • HPLC with diode array detector
  • UV/VIS spectrophotometer

Routine Procedures:

  • Flavor chemistry procedures

Contact Person(s):
Luke Boatright 7-5988

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Ruminant Nutrition Lab

Major Equipment:

  • UV/VIS spectrophotometer
  • COBAS centrifugal analyzer
  • Atomic absorption spectrophotometer (includes graphite furnace)
  • HPLC with UV/VIS and fluorescent detection
  • LECO N-analyzer
  • Gas chromatograph with FID
  • Standard and Ankom fiber analysis equipment

Routine Procedures:

Metabolites

  • Ruminal Volatile Fatty Acid (VFA)
  • Blood VFA
  • D- And L-Lactate (Enzymatic)
  • Lactic Acid (Barker Summerson)
  • Pyruvate Analysis
  • Acetoacetate Analysis
  • b-Hydroxybutyrate Analysis
  • L-Alanine Analysis
  • Glutamate Analysis
  • Glutamine Analysis
  • Free Fatty Acid Analysis
  • Vitamin E In Plasma
  • Blood Glucose Specific Activity

Enzymes

  • Amylase
  • Trypsin
  • Chymotrypsin
  • Carboxypeptidase A
  • Ruminal Fluid Urease Assay

Nitrogen

  • Kjeldahl Nitrogen And Protein
  • Acid Detergent Insoluble Nitrogen
  • Pepsin Insoluble Nitrogen
  • Protein Assay Using Bca (Pierce, No. 23225)
  • Ammonia

Fiber

  • Total Dietary Fiber In Foods
  • Neutral Detergent Fiber (Cell Wall Constituents)
  • Alternative Procedure For High Starch Samples
  • Acid Detergent Fiber (ADF)
  • Acid Detergent Lignin (ADL)
  • Filtrations Using Filter Paper For (ADF and NDF )
  • Crucible Cleaning Procedure
  • Ankom Method For ADF
  • Ankom Method For NDF
  • Ankom Method For Crude Fiber
  • Ankom Method For ADL Using Beakers

    Markers

  • Acid Insoluble Ash
  • Atomic Absorption Analysis Of Chromic Oxide
  • Analysis Of Chromic Oxide By Wet Ashing
  • Chromic Oxide-Dry Ashing And Colorimetric
  • DTPA Extraction For Rare-Earth Element Analysis
  • Preparation Of Chromium And Cobalt EDTA
  • Indigestible Adf (IADF)

Other Analyses

  • Starch Analysis
  • Total Long Chain Fatty Acid Analysis
  • Analysis Of Total Lipid Content
  • Inorganic Phosphate Determination
  • Total Calcium Analysis
  • Brush Border Membrane Vesicles
  • Maltase Assay For Transport
  • Alkaline Phosphatase Assay For Transport

    Microbial Analyses

  • Separation Of Bacteria And Protozoa
  • Bacterial Isolation From Rumen Contents
  • Determination Of D-Alanine
  • Purine Content Of Ruminal Microbes And Digesta
  • Tilley-Terry Two Stage In Vitro Fermentation
  • Van Soest Modification Of Tilley-Terry Procedure
  • Ankom In Vitro True Digestibility

    Cobas Procedures

  • Measuring Absorbance At 280 Nm
  • Alpha Amino Nitrogen
  • Alpha Amino Nitrogen (Hydrolysates)
  • Plasma Amylase
  • Ammonia Nitrogen Using Gdh For Plasma
  • Ammonia Nitrogen Using Salicylate
  • Bca Soluble Protein
  • b-Hydroxybutyrate In Blood
  • Cholesterol In Plasma
  • Creatinine For Plasma And Urine
  • Chymotrypsin In Pancreatic Juice
  • Plasma Glucose Using Hexokinase
  • Inorganic Phosphorus
  • Free Fatty Acids In Plasma
  • Pah Analysis
  • Trypsin In Bovine Pancreatic Juice

Contact Person(s):
David L. Harmon 7-7516
Dani True 7-2977

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