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Course Syllabus for Fall 2012

Lecture: Tuesday and Thursday, 8:00 - 8:50 am; 109 Garrigus
Laboratory: Tuesday and Thursday, 9:00 - 10:50 am; 105 Garrigus
Luke Boatright, Ph.D.
Office: 412 Garrigus
Phone: 257-5988
E-mail: wlboat1@uky.edu Office Hours: Open


"Food Analysis, 4th edition," S.S. Nielsen, Ed., 2010. Springer, New York, NY.


Evaluation Cumulative Numerical Grade Letter Grade
Quizzes 10% Undergraduates Graduates
Exams 50% 90-100 92-100 A
Lab Reports 25% 80-89 82-91 B
Individual Projects 10% 70-79 72-81 C
Class Participation 5% 60-69 62-71 D
100% Below 60

Below 62


The numerical scale given here will be the guideline for assigning final grades in this course. The numerical scale may or may not be lowered in assigning the final grades, but will not be any higher than that indicated. 

A quiz will be given about every week. The lowest quiz score will be discarded. The four exams will cover both lecture and lab material. The final exam, which is not comprehensive, is optional for undergraduate students.

Subject mater is best retained when students participate in classroom discussions and ask questions. In order to effectively participate in these discussions each reading assignment should be completed prior to the corresponding class period.   Five percent of a students final grade will reflect their involvement in classroom discussions. 

Missed quizzes and exams can be made up only if: a) Notification is given in advance of a justifiable absence, or b) An unanticipated, justifiable absence is verified.  In accordance with the University rules governing absences, as provided by the University Senate Rules Sections V - 2.4.1 and 2.4.2 (http://www.uky.edu/StudentAffairs/Code/part2.html), an excess of each three (3) unexcused absences for lectures will result in a drop in the final letter grade for the class. Unexcused laboratory absences can not be made-up and will count as a zero (0) for that laboratory write-up. If a student has excussed absences in excess of one-fifth of the class contact hours, the student will be required to withdraw from the course (University Senate Rules Section V-

Instructions for the individual project are given on a separate sheet (note: graduate students cannot use any topic relating to their thesis for their class project). All assignments submitted by students should represent their own work and ideas unless appropriate recognition is given to the original author. University policies related to plagiarism can be found in your copy of Student Rights and Responsibilities or at http://www.uky.edu/StudentAffairs/Code/part2.html section 6.3.1.  Any student whom the instructor has sufficient evidence to believe has cheated or plagiarized in the course will receive an automatic "E" (failure) in the entire course. There will be no exceptions.

Students will be informed of their current progress based on the criteria in the syllabus before the midterm date of the semester, term or session. (SR 6.1.3.A)

Students should provide one week notice to the instructor in order to arrange for accommodations due to a religious observance.


Chapters in the required textbook will be assigned for each lecture. Students are expected to read the assignment and be prepared to ask questions and discuss the material in lecture.  Important course information is often discussed at the beginning of lecture, so please do not be late. There will be discussion in lecture and lab concerning the laboratory experiments.  The laboratory procedures should be brought to lecture so they can be discussed during class before each lab. Students are to arrive at the lab well prepared to do the experiment. Some aspects of the experiment done in lab (e.g. Questions for Class Discussion from laboratory handout) will be discussed in the following lecture.


Official Methods of Analysis. Association of Official Analytical Chemists, 15th ed. (1990).
(on reserve or in reference section of the Agriculture Library).
Official Methods and Recommended Practices, American Oil Chemists' Society, 4th ed.(1987).
Food Analysis: Theory and Practice. Pomeranz and Meloan, 3rd. ed., (1994).
Food Chemistry. Fennema, (1985).
Food Analysis: Principles and Techniques. Gruenwedel and Whitaker, Vol. 1 (1984), Vol 2, (1984).
Food Composition and Analysis. Aurand, Woods and Wells, (1987).
The Merck Index. (in reference section of Agriculture Library)
Code of Federal Regulations (CFR) 21:Parts 100-169; 9: Parts 200-319.(http://www.access.gpo.gov/nara/cfr/cfr-table-search.html)
Nutritive Value of American Foods. USDA Agriculture Handbook, (1984).
Food composition and nutritional tables, CRC Press, (1994).


Application of quantitative and qualitative analysis used in the physical, chemical and instrumental examination of food products. A special emphasis is placed on the evaluation of methods and interpretation of results.


To identify the principles, purposes, and applications of techniques to the chemical and instrumental analysis of  foods.
To identify appropriate methods for proximate analysis of food products.

To provide chemical and instrumental laboratory experience for students in the Food Science curriculum.


Food Analysis (FSC 535)
Tuesday and Thursday; Class, 8:00 - 8:50
Laboratory, 9:00 - 10:50

Lecture Topics

Date Description Reading
August 23 Introduction
August 28 Titratable Acidity Ch. 13
August 30 Principles of Ultraviolet, Visible and Fluorescence Spectroscopy Ch. 22, 23
September 4 Moisture and Ash Ch. 6, 7
September 6 Mineral Analysis Ch. 12, 25
September 11 Principles of Chromatography Ch. 27
September 13 Liquid Chromatography Ch. 28
September 18 Gas-Liquid Chromatography Ch. 29
September 20 Oven Review
September 25 Exam I
September 27 Carbohydrate Analysis Ch. 10
October 2 Carbohydrate Analysis (Con't)
October 4 Carbohydrate Analysis (Con't)
October 9 Lipid Analysis Ch. 8, 14
October 11 Lipid Analysis (Con't)
October 16 Lipid Analysis (Con't)
October 18 Open Review
October 23 Exam II
October 25 Protein Analysis Ch. 9, 15
October 30 Protein Separation & Characterization
November 1 Protein Separation & Characterization
November 6 Protein Analysis Continued / Reading Assisgnment
November 8 Vitamins
November 13 Pigments Ch. 11 & 32
November 15 Open Review
November 20 Exam III
November 22 Holiday Ch. 26
November 27 Mass Spectrometry
November 29 Sensory Analysis
December 4 Immunoassays & Thermal Analysis Ch. 17, 31
December 6 Open Review
Final Exam, (IV) - Wednesday, December 12, 8:00 A.M. 


 Laboratory Schedule
Date Description Report Due Date
August 23 Introduction and Searching the Literature
August 28 Standard Solutions and Titratable Acidity Sept. 11
August 30 Principles of Spectroscopy, Dilutions and Standard Curves Sept. 11
September 4 Moisture and Total Ash Sept. 18
September 6 Traditional Mineral Analysis Sept. 18
September 11 Thin-Layer Chromatography Sept. 27
September 13 Liquid Chromatography Sept. 27
September 18 Gas-Liquid Chromatography Sept. 27
September 20 Individual Projects
September 25 Exam I
September 27 Carbohydrates Analysis Oct. 11
October 2 Carbohydrate Analysis Continued Oct. 11
October 4 Vitamin Analysis - Library Search Oct. 18
October 9 Lipid Analysis (Extract &FFA) Oct. 25
October 11 Lipid Analysis (Extract & Phos) Oct. 25
October 16 Lipid Oxidation Oct. 25
October 18 Individual Projects
October 23 Exam II
October 25 Protein Analysis Nov. 15
October 30 Protein Separation & Characterization
November 1 Protein Separation & Characterization Nov. 15
November 6 Vitamin Analysis I Nov. 22
November 8 Vitamin Analysis II
November 13 Pigment Analysis Nov. 27
November 15 Individual Projects
November 20 Exam III
November 22 Holiday
November 27 Mass Spectrometry Dec. 6
November 29 Sensory Analysis Dec. 6
December 4 Individual Projects
December 6 Presentation of Individual Projects
*Reports with same due date can be combined into one report