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Click here for our old rates (July 2007 - June 2009) | |
| Effective July 2010 - June 2011 | |
| Our flexible menu of schedules allows clients to customize their projects in a modular approach. This approach works well with Sanger sequencing projcts and pyrosequencing projects. For instance, one unit of Schedule 12 plus two units of Schedule 7 combine to make a “Cells-to-Sequences, Forward and Reverse Primers” type project. Please note it is advised that clients meet with AGTC staff to plan a pyrosequencing project. | |
I. Sequencing Schedules |
||||
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
| Schedule
1 -- Run only Individual Sequencing Product on ABI 3730 short description |
sample |
Client prepares and purifies single Big Dye Reactions. |
$3.22 |
$3.22 |
|
Schedule 3 -- GS Titanium full plate run short description |
plate |
Plate may be divided into 2,4,8,and 16 sections and the cost distributed among the samples. |
$4,471.67 |
$4,471.67 |
|
Schedule 4 -- Run Only 95 Sequencing Products on ABI 3730 short description |
96-well plate |
AB Microamp plates are supplied to the client at no additional charge. Please leave well number A01 empty for standard. |
$41.06 |
$0.43 |
|
Schedule 5 -- React & Run -- Sequencing of individual DNA templates (1-13 samples) with optional QA short description |
sample |
PicoGreen quant may be done to select optimum template volume to react. Customer will be charged for one control with each submission. |
$13.67 |
$13.67 |
|
Schedule 6 -- React & Run plasmid or PCR products with optional QA -- Sequencing of 14-94 DNA templates short description |
96-well plate |
AB Microamp plates are supplied to the client at no additional charge. PicoGreen quant may be performed to select optimum template volume. Please have wells A01 and B01 empty. |
$180.75 |
$1.92 |
|
Schedule 7 -- React & Run High Throughput of plasmids with QA -- Sequencing of 752 (8x94) DNA templates short description |
8x96 well plates |
QA is necessary in initial phases of a high throughput project. Sequencing may switch to Schedule 7A after sample quality has been established. Number of plates may be combined with 7A to qualify for high throughput prices. |
$1,382.16 |
$1.84 |
|
Schedule 7A -- React & Run High Throughput of plasmids without QA -- Sequencing of 752 (8x94) DNA templates short description |
8x96 well plates |
Sample quality has been established and QA is not necessary. Number of plates may be paired with Schedule7 to qualify for high throughput prices. |
$955.53 |
$1.27 |
|
Schedule 8 -- React & Run High Throughput of cosmids or fosmids with QA -- Sequencing of 752 (8x94) DNA templates short description |
768 wells (8x96 well plate) |
Same as Schedule 7 except for cosmids and fosmids. |
$1,756.22 |
$2.34 |
|
Schedule 8A -- React & Run High Throughput of cosmids or fosmids without QA -- Sequencing of 752 (8x96) DNA templates short description |
8x96 well plates |
Sample quality has been established and QA is not necessary. Number of plates may be paired with Schedule 8 to qualify for high throughput prices. |
$1,315.20 |
$1.75 |
|
Schedule 9 -- Run only Fragment Analysis, up to 96 wells short description |
96 well plate |
AGTC will run fragment analysis samples (microsatellite, TFRLP, SnapShot, etc) on 3730. Client must do reactions and analyze samples. |
$46.64 |
$0.49 |
|
Schedule 26 -- Additional primers short description |
96 well plate |
Only applies to Schedule 6. |
$4.95 |
n/a |
II. DNA Isolation Schedules |
||||
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
|
Schedule 11 -- Plasmid DNA Purification from Bacteria with
Optional QA (single 96-well plate) short description |
1x96-well plates |
-
|
$133.14 |
$1.42 |
| Schedule 12 -- High throughput Plasmid DNA Purification from Bacteria -- 752 clones (8x96-well plates) |
8x96-well plates |
High throuput version of Schedule 11. QA is performed in the sequencing step.
|
$1,145.26 |
$1.52 |
| Schedule 13 -- High throughput fosmid Purification from Bacteria with QA -- 752 clones (8x94-well plates) |
8x96-well plates |
Same as Schedule 12 except for fosmids or cosmids.
|
$1,152.84 |
$1.53 |
III. Sequencing Reaction Clean-up Schedules |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
|
Schedule 10 --
Sequence Reaction Clean-up with ethanol precipitation, single samples.
short description |
1-13 samples |
Standard sequencing clean-up for Schedule 1 samples.
|
$0.59 |
n/a |
| Schedule 10A -- Sequence Reaction Clean-up with ethanol precipitaion, one 96-well plate |
96-well plate |
Standard sequencing clean-up for Schedule 4 samples.
|
$4.26 |
$0.04 |
| Schedule 20 -- Sequencing reaction clean-up with AB BigDye Xterminator, individual samples |
sample |
May improve reads near dye front and for low concentration template. |
$1.34 |
$1.34 |
IV. PCR Product Purification Schedules |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
|
Schedule 15 -- PCR Product Purification of up to 94 samples using magnetic beads. |
sample |
Charge is prorated to number of samples. |
$73.96 |
$0.78 |
|
Schedule 23 -- Alternative PCR Purification method, enzymatic clean-up of 1-95 samples. |
sample |
Charge is prorated to number of samples |
$89.50 |
$0.94 |
V. Other Services |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
|
Schedule 16 -- Nylon Membrane Gridding (MacroArraying) of Bacterial or DNA Samples short description |
96-well plate |
- |
$230.73 |
Varies |
|
Schedule 21 -- AGTC Facility Equipment usage by staff or client short description |
Hour |
$8.40 |
n/a |
|
|
Schedule 22 -- Robotic Programming by Staff for Client Project |
Hour |
Programming of robots or instruments by AGTC Staff for client-specific custom protocols |
$18.29 |
n/a |
| Schedule
24 -- Gel Electrophoresis |
Gel of a 96 well plate. |
- |
$21.30 |
n/a |
| Schedule
25 -- dsDNA quant |
96 well plate |
- |
$22.40 |
$0.24 |
| Schedule
27 -- Copies of cell cultures in 96 or 384 well plates |
2 copies of 8x384 well plates. |
Biomek robot used to copy cultures for storage in freezer medium. Recommended for plasmid, fosmid or cosmid libraries. | $104.87 |
$0.03 / sample |
| Schedule
28 -- Load and run TLDA cards. short description |
TLDA card |
Run on AB HT 7900 Real-time PCR | $33.48 |
$33.48 /TLDA card |
VI. Use of Equipment by Client |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
| Schedule
17 -- HiGro Use by Client short description |
Hour |
Client should be trained on use, particularily balancing plates. | $12.21 |
n/a |
| Schedule
18 -- Use of Thermalcycler by Client per hour short description |
Hour |
If time is not logged, an overnight run of 16hrs will be assumed |
$4.88 |
n/a |
| Schedule
19 -- Non-Robotic Equipment Useage by Client per hour |
Hour |
Operation or programming of non-robotic equipment by registered Users (e.g. Centrifuge, Hydroshear, Qfill), Client resposible for clean-up (otherwise facility will charge 3 additional units). | $3.98 |
n/a |
| Schedule
29 -- Use of the GenoGrinder by Client |
Use |
Each use is one unit. Client MUST balance tubes. | $17.34 |
n/a |
VII. 454 Related Schedules |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
| Schedule
30 -- Library preparation, whole genome, for shotgun 454 sequencing short description |
sample |
- |
$795.30 |
$795.30 |
| Schedule
31 -- Small volume emulsion PCR sample preperation for titration or small volume 454 run. Four seperate emulsions are prepared. short description |
sample |
- |
$914.52 |
$914.52 |
| Schedule
32 -- Large volume emulsion PCR for 1/2 or whole plate run short description |
sample |
Medium volume emPCR is 1/8 whole large vloume. |
$2,159.48 |
$2,159.48 |
| Schedule
33 -- cDNA library with directional adapters |
sample |
Construction of a cDNA library with directional adapters for 454 sequencing. |
$1,527.07 |
$1,527.07 |
| Schedule
34 -- Paired-end library, 3kb |
sample |
Preparation of a paired end library of 3kb fragments for 454 sequencing. |
$1,155.02 |
$1,155.02 |
| Schedule
34A -- Paired-end library, 8kb |
sample |
Preparation of a paired end library of 8kb fragments for 454 sequencing. |
$1,327.46 |
$1,327.46 |
| Schedule
34B -- Paired-end library, 20kb |
sample |
Preparation of a paired end library of 20kb fragments for 454 sequencing. |
$1,392.87 |
$1,392.87 |
Last updated: 30 June, 2010
UK-AGTC