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Click here for our old rates (July 2007 - June 2009) | |
| Effective July 2009 - June 2010 | |
| Our flexible menu of schedules allows clients to customize their projects in a modular approach. For instance, one unit of Schedule 12 plus two units of Schedule 7 combine to make a “Cells-to-Sequences, Forward and Reverse Primers” type project. Or, if a client wished to have their bacterial library gridded, probed, cherry-picked for interesting clones, then had those interesting clones sequenced, that project would consist of Schedules 16, 21, and then 12+7 or 11+6 depending on the number of clones sequenced. For info on how to register your lab and projects with AGTC, click here. | |
I. Sequencing Schedules |
||||
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
| Schedule
1 -- "Run Only" Individual Sequencing Product on ABI 3730 short description |
sample |
- |
$4.64 |
$4.64 |
|
Schedule 2 -- GS FLX Titanium Titration plate short description |
1/4 plate |
To keep cost low customers may have to wait until 4 libraries are ready to run at once. Usable sequencing data will be obtained from this run. |
$1,659.16 |
$1,659.16 /4 lanes |
|
Schedule 3 -- GS Titanium full plate run short description |
plate |
Plate may be devided into 2,4,8,and 16 sections and the cost ditributed among the samples. |
$6,223.18 |
$6,223.18 |
|
Schedule 4 -- "Run Only" 96 Sequencing Products on ABI 3730 short description |
96-well plate |
AB Microamp plates are supplied to the client at no additional charge. |
$91.87 |
$0.97 |
|
Schedule 4A -- High Throughput "Run Only" 96 Sequencing Products on ABI 3730 short description |
8x96-well plates delivered at the same time |
AB Microamp plates are supplied to the client at no additional charge. |
$710.64 |
$710.64 |
|
Schedule 5 -- "React & Run" -- Sequencing of individual DNA templates (1-17 samples) with optional QA short description |
sample |
SybrGold quant may be done to select optimum template volume to react. Customer will be charged for one control with each submission. |
$14.64 |
$14.64 |
|
Schedule 6 -- "React & Run" plasmid or PCR products with optional QA -- Sequencing of 18-96 DNA templates short description |
96-well plate |
AB Microamp plates are supplied to the client at no additional charge. SybrGold quant may be performed to select optimum template volume. |
$242.76 |
$2.58 |
|
Schedule 6A -- "React & Run" fosmid or cosmid products with optional QA -- Sequencing of 18-96 DNA templates short description |
96-well plate |
AB Microamp plates are supplied to the client at no additional charge. SybrGold quant may be performed to select optimum template volume. 20ul reactions, 100 cycles. |
$280.25 |
$2.98 |
|
Schedule 7 -- "React & Run" High Throughput of plasmids with QA -- Sequencing of 752 (8x94) DNA templates short description |
768 wells (8x96 well plate) |
QA is necessary in initial phases of a high throughput project. Sequencing may switch to Schedule 7A after sample quality has been established. Number of plates may be combined with 7A to qualify for high throughput prices. |
$1,328.68 |
$1.77 |
|
Schedule 7A -- "React & Run" High Throughput of plasmids without QA -- Sequencing of 752 (8x94) DNA templates short description |
768 wells (8x96 well plate) |
Sample quality has been established and QA is not necessary. Number of plates may be paired with Schedule7 to qualify for high throughput prices. |
$1,004.58 |
$1.34 |
|
Schedule 8 -- "React & Run" High Throughput of cosmids or fosmids with QA -- Sequencing of 752 (8x94) DNA templates short description |
768 wells (8x96 well plate) |
Templates may be provided by customer or may be products of another AGTC schedule (may be paired with Schedule 13). QA is necessary in initial phases of a high throughput project. Sequencing may switch to Schedule 8A after sample quality has been established. |
$1,645.29 |
$2.19 |
|
Schedule 8A -- "React & Run" High Throughput of cosmids or fosmids without QA -- Sequencing of 768 (8x96) DNA templates short description |
768 wells (8x96 well plate) |
Sample quality has been established and QA is not necessary. Number of plates may be paired with Schedule 8 to qualify for high throughput prices. |
$1,321.28 |
$1.72 |
|
Schedule 9 -- "Run only" Fragment Analysis, up to 96 wells short description |
96 well plate |
AGTC will run fragment analysis samples (microsatellite, TFRLP, SnapShot, etc) on 3730. Client must do reactions and analyze samples. |
$55.81 |
$0.58 |
|
Schedule 26 -- Additional primers short description |
96 well plate |
Only applies to Schedules 6, 6A, 7, 7A, 8, and 8A. |
$5.52 |
n/a |
II. DNA Isolation Schedules |
||||
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
|
Schedule 11 -- Plasmid DNA Purification from Bacteria with
Optional QA (single 96-well plate) short description |
(1x96-well plates) |
-
|
$246.61 |
$2.62 |
|
Schedule 11A -- Fosmid or cosmid DNA Purification from Bacteria with
Optional QA (single 96-well plate) short description |
(1x96-well plates) |
-
|
$257.02 |
$2.73 |
|
Schedule 12 -- High throughput Plasmid DNA Purification from
Bacteria -- 752 clones (8x94-well plates)
short description |
768 wells (8x96-well plates) |
-
|
$1,281.37 |
$1.70 |
|
Schedule 13 -- High throughput fosmid Purification from
Bacteria with QA -- 752 clones (8x94-well plates)
short description |
768 wells (8x96-well plates) |
-
|
$1,298.29 |
$1.73 |
III. Sequencing Reaction Clean-up Schedules |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
|
Schedule 10 --
Sequence Reaction Clean-up with ethanol precipitation, single samples.
short description |
1-20 samples |
-
|
$0.41 |
$0.02 |
|
Schedule 10A --
Sequence Reaction Clean-up with ethanol precipitaion, one 96-well plate
short description |
96-well plate |
-
|
$2.19 |
$0.02 |
|
Schedule 20 --
Sequencing reaction clean-up with AB BigDye Xterminator, individual samples
short description |
sample |
May improve reads near dye front and for low concentration template. |
$1.57 |
$1.57 |
|
Schedule 20A --
Sequencing reaction clean-up with AB BigDye Xterminator, 96-well plate
short description |
96-well plate |
May improve reads near dye front and for low concentration template. |
$124.53 |
$1.31 |
IV. PCR Product Purification Schedules |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
|
Schedule 15 -- PCR Product Purification with optional
QA -- 1-24 samples short description |
sample |
Uses individual purification colunms. |
$4.51 |
$0.19 |
|
Schedule 15A -- PCR Product Purification with optional
QA -- 25-96 samples short description |
sample |
Solid-phase or filtration purification in 96 well format. |
$99.75 |
$1.05 |
|
Schedule 23A -- Alternative PCR Purification method, magnetic beads short description |
sample |
Good for samples containing extraneous materials, such as BSA or dyes. |
$123.83 |
$1.30 |
|
Schedule 23B -- Alternative PCR Purification method, enzymatic clean-up short description |
sample |
Excellent recovery. Good for fragments as low as 125bp. |
$133.89 |
$1.41 |
V. Other Services |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
|
Schedule 16 -- Nylon Membrane Gridding (MacroArraying) of Bacterial or DNA Samples short description |
96-well plate |
- |
$285.43 |
Varies |
|
Schedule 21 -- Nylon Membrane Gridding (MacroArraying) of Bacterial or DNA Samples short description |
- |
In some cases, client assistance will be permitted to reduce the time and therefore, the cost. |
$5.51 |
n/a |
|
Schedule 22 -- Robotic Programming by Staff for Client Project short description |
- |
- |
$19.80 |
n/a |
| Schedule
24 -- Gel Electrophoresis short description |
Gel |
- |
$25.64 |
$0.03 |
| Schedule
25 -- SybriGold Quant short description |
24 samples |
- |
$30.85 |
$1.29 |
| Schedule
27 -- Copies of cell cultures in 96 or 384 well plates short description |
- |
Biomek robot used to copy cultures for storage. Recommended for plasmid, fosmid or cosmid libraries. | $129.38 |
$8.08 /plate |
| Schedule
28 -- Load and run TLDA cards. short description |
- |
Run on AB HT 7900 Real-time PCR | $39.98 |
$39.98 /TLDA card |
VI. Use of Equipment by Client |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
| Schedule
17 -- HiGro Use by Client short description |
0.1 hour |
Client should be trained on use, particularily balancing plates. | $15.56 |
n/a |
| Schedule
18 -- Use of Thermalcycler by Client short description |
0.1 hour |
If time is not logged, an overnight run of 16hrs will be assumed |
$6.20 |
n/a |
| Schedule
19 -- Non-Robotic Equipment Useage by Client short description |
- |
Operation or programming of non-Biomek equipment by registered Users (e.g. Centrifuge, Hydroshear, Qfill), Client resposible for clean-up (otherwise facility will charge 3 additional units). | $1.37 |
n/a |
| Schedule
29 -- Use of the GenoGrinder by Client short description |
- |
Each use is one unit. Client MUST balance tubes. | $17.40 |
n/a |
VII. 454 Related Schedules |
| Schedule
Name |
Schedule
Unit of Activity |
Notes
|
Fee/ Schedule Unit |
Cost/ Well or Sample |
| Schedule
30 -- Library preparation, whole genome, for shotgun sequencing short description |
sample |
- |
$1,020.40 |
$1,020.40 |
| Schedule
31 -- Small volume emusion PCR sample preperation for titration or small volume 454 run. short description |
sample |
- |
$886.81 |
$886.81 |
| Schedule
32 -- Large volume emusion PCR for 1/2 or whole plate run short description |
sample |
- |
$2,837.10 |
$2,837.10 |
Last updated: 29 July, 2009
UK-AGTC