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NCBI
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GCG NUTS and BOLTS
There are a few GCG utilities that can help make manipulations MUCh
easier.
Sequence Input/Editing
GETSEQ: copy sequence from anywhere, paste it into a GCG format
file
SEQED: edit a GCG format file (beware, this is the vi editor
thinly veiled)
Getting Output
Sequence Output
MAP is a handy way to output a sequence and to examine its
translations
PUBLISH is a way to get a publication quality output that is very
flexible
MAPSORT/MAPPLOT is the way to get a graphic output of a restriction map
Sequence Manipulation
REVERSE can do reverse complement, but it can also do
reverse (in the event that you ever enter a sequence 3'-5' by accident)
SHUFFLE randomizes sequences, which can be handy in alignment
comparisons, etc.
COMPOSITION tells you the nucleotides, dinucleotides, and trinucleotides
or amino acids
FINDPATTERNS is a great search utility on a sequence
Reformat functions include FromGenbank, FromFASTA, FromEMBL and ToFASTA,
etc.
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