DNA Sequencing

11/5/98


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Table of Contents

DNA Sequencing

Project Size/Type

DNA Sequencing Methods

Dideoxy/Chain Terminator/Sanger

Chain Terminator Basics

Electrophoresis

Template Preparation

Primers

Extension Chemistry

Separation

Paradigm Instrument

Alternate Instruments

Sample Output

Trace Editing

Project Goals

Sequencing Strategies

Random Method

Random

Poisson Statistics

Poisson-2

Poisson-3

4 Mbp Genome

3000 Mbp Genome HUMAN

Automation

Costs

Ordered Methods

Limitations

Finishing

Finish-How

Assembly Methods

Assembly Programs

Assembly Improvements Repeat Problems

Project Management

Annotation

Sequencing by Hybridization

Modern Sequencing Challenges

Physical Mapping Genome Characterization

Author: cstaben

Email: staben@pop.uky.edu

Home Page: www.uky.edu/Classes/BIO520/BIO520WWW/

Other information:
Lab9 will be up very soon.

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