Genome Characterization

Table of Contents

Genome Characterization

Assembly Problem

Contigs, Islands

Assembly Methods

Assembly Programs

Project Management

Annotation

Sequencing by Hybridization

Modern Sequencing Challenges

Physical Mapping

Physical Mapping

Clone-based Maps

Genome Fragmentation

Separation of Large DNAs

PFGE

Cloning Vectors

YAC

YACs, cont’d

BACs, PACs

Cosmids,?, plasmids

M13, phagemids, “physical” clones

Overlaps/Assembly

Hybridization

Fingerprinting

Assembly ImprovementsRepeat Problems

Email: staben@pop.uky.edu

Home Page: www.uky.edu/Classes/BIO520/BIO520WWW/

Other information:
Lab10 will be up very soon.

Best experienced with

Click here to start.

Download presentation source