!!NA_SEQUENCE 1.0
LOCUS       BOVMG        1077 bp ss-RNA             MAM       19-JUN-1989
DEFINITION  Bovine myoglobin mRNA.
ACCESSION   D00409
NID         g217579
KEYWORDS    myoglobin.
SOURCE      Fetal bovine skeletal muscle, cDNA to mRNA.
  ORGANISM  Bos taurus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
            Vertebrata; Mammalia; Eutheria; Artiodactyla; Ruminantia; Pecora;
            Bovoidea; Bovidae; Bovinae; Bos.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Shimada,H., Fukasawa,T. and Ishimura,Y.
  TITLE     Expression of bovine myoglobin cDNA as a functionally active
            holoprotein in Saccharomyces cerevisiae
  JOURNAL   J. Biochem. 105 (3), 417-422 (1989)
  MEDLINE   89278066
COMMENT     The complete coding sequence of myoglobin as well as the 3'- and
            part of the 5'-nontranslatable sequences were determined.  The
            amino acid sequence predicted from the nucleotide sequence is
            different from the reported amino acid sequence of myoglobin
            purified from the adult bovine cardiac muscle at eight locations.
            An expression system in Saccharomyces cerevisiae was established.
FEATURES             Location/Qualifiers
     source          1. .1077
                     /organism="Bos taurus"
                     /db_xref="taxon:9913"
     mRNA            <1. .1077
                     /note="myoglobin mRNA"
     CDS             67. .531
                     /note="myoglobin precursor"
                     /codon_start=1
                     /db_xref="PID:d1000763"
                     /db_xref="PID:g217580"
                     /translation="MGLSDGEWQLVLNAWGKVEADVAGHGQEVLIRLFTGHPETLEKF
                     DKFKHLKTEAEMKASEDLKKHGNTVLTALGGILKKKGHHEAEVKHLAESHANKHKIPV
                     KYLEFISDAIIHVLHAKHPSDFGADAQAAMSKALELFRNDMAAQYKVLGFHG"
     mat_peptide     <70. .>528
                     /note="myoglobin mature protein"
     polyA_signal    1058. .1063
BASE COUNT      246 a    291 c    311 g    229 t
ORIGIN      3 bp upstream of PvuII site.
 
bovmg.seq  Length: 1077  September 26, 1998 15:54  Type: N  Check: 2311  ..

       1  CAGCTGTCGG AGACAGACAC CCAGTCAGTC CCGCCCTTGT TCTTTTTCTC 

      51  TTCTTCAGAC TGCGCCATGG GGCTCAGCGA CGGGGAATGG CAGTTGGTGC 

     101  TGAATGCCTG GGGGAAGGTG GAGGCTGATG TCGCAGGCCA TGGGCAGGAG 

     151  GTCCTCATCA GGCTCTTCAC AGGTCATCCC GAGACCCTGG AGAAATTTGA 

     201  CAAGTTCAAG CACCTGAAGA CAGAGGCTGA GATGAAGGCC TCCGAGGACC 

     251  TGAAGAAGCA TGGCAACACG GTGCTCACGG CCCTGGGGGG TATCCTGAAG 

     301  AAAAAGGGTC ACCATGAGGC AGAGGTGAAG CACCTGGCCG AGTCACATGC 

     351  CAACAAGCAC AAGATCCCTG TCAAGTACCT GGAGTTCATC TCGGACGCCA 

     401  TCATCCATGT TCTACATGCC AAGCATCCTT CAGACTTCGG TGCTGATGCC 

     451  CAGGCTGCCA TGAGCAAGGC CCTGGAACTG TTCCGGAATG ACATGGCTGC 

     501  CCAGTACAAG GTGCTGGGCT TCCATGGCTA AGCCCCACCC CTGTGCCCCT 

     551  CACCCCACCC ACCTGGGCAG GGTGGGCGGG GACTGAATCC CAAGTAGTTA 

     601  TAGGGTTTGC TTCTGAGTGT GTGCTTTGTT TAGGAGAGGT GGGTGGAAGA 

     651  GGTGGATGGG TTAGGGGTGG AGGGAGCCTT GGGAGAGGCC TGGGGACCAG 

     701  GCTTTCAGTG GAGGGTGCAT CAACTTGGGA ACCATGAGAA GCTTGACTGT 

     751  GGCTGGCTGA GTCTGGGTCA AACTCAACTT TCCTTTCACC TCAATGCCAA 

     801  CCCAATTCCT ACCAACCTCT AAACTGACCT GCACCTTTAC CCTCACCTTA 

     851  AATCCCCAAT CCGAGCTGTC AACATAAACT CCAGCCTAAT TCTCTGACCC 

     901  CATCACCCAG CCCCTTGAAG ACAGCAGAGT GTCTTGCTTG CCCTGAGAAG 

     951  GAAGTGTGGG CCGGGTGGGA CGGCCACACC CAGCCCTAGG GAGGCATGGA 

    1001  GGCATGGTGT CTGCAACATA AATGTCCCTT CTCAGGTAGG GGAGTGACAC 

    1051  CTGGTTTAAT AAAGGATTTC TCACATC