!!AA_SEQUENCE 1.0 LOCUS 217580 154 aa 19-JUN-1989 DEFINITION myoglobin precursor. ACCESSION 217580 PID g217580 DBSOURCE DDBJ: locus BOVMG, accession D00409 KEYWORDS . SOURCE Bos taurus. ORGANISM Bos taurus Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata; Vertebrata; Mammalia; Eutheria; Artiodactyla; Ruminantia; Pecora; Bovoidea; Bovidae; Bovinae; Bos. REFERENCE 1 (residues 1 to 154) AUTHORS Shimada,H., Fukasawa,T. and Ishimura,Y. TITLE Expression of bovine myoglobin cDNA as a functionally active holoprotein in Saccharomyces cerevisiae JOURNAL J. Biochem. 105 (3), 417-422 (1989) MEDLINE 89278066 COMMENT The complete coding sequence of myoglobin as well as the 3'- and part of the 5'-nontranslatable sequences were determined. The amino acid sequence predicted from the nucleotide sequence is different from the reported amino acid sequence of myoglobin purified from the adult bovine cardiac muscle at eight locations. An expression system in Saccharomyces cerevisiae was established. FEATURES Location/Qualifiers source 1. .154 /organism="Bos taurus" /db_xref="taxon:9913" Protein 1. .154 /name="myoglobin precursor" mat_peptide <2. .>154 /note="myoglobin mature protein" /codon_start=1 CDS 1. .154 /coded_by="D00409:67. .531" ORIGIN 217580.pep Length: 154 September 26, 1998 15:53 Type: P Check: 5708 .. 1 MGLSDGEWQL VLNAWGKVEA DVAGHGQEVL IRLFTGHPET LEKFDKFKHL 51 KTEAEMKASE DLKKHGNTVL TALGGILKKK GHHEAEVKHL AESHANKHKI 101 PVKYLEFISD AIIHVLHAKH PSDFGADAQA AMSKALELFR NDMAAQYKVL 151 GFHG