The transgenic mouse provides an excellent model system in which to study the complexities of mammalian development and cell differentiation as well as genetically linked human diseases. To provide the University of Kentucky research community with a centralized facility for the generation of transgenic mice, the Transgenic Facility was established in 1989. The facility provides state-of-the-art equipment and expertise essential for producing transgenics and maintains a mouse colony in the UK Division of Laboratory Animal Research facility that is dedicated to transgenic production. The microinjection of purified DNA and all surgical procedures are conducted by a full-time research analyst.
The transgenic laboratory is located in the Dorothy E. Combs Cancer Research Building of the Lucille P. Markey Cancer Center. The laboratory is equipped with a Nikon Diaphot TMD microscope with Hoffman modulation contrast optic system, two Narashige micromanipulators, Nikon stereomicroscopes used for surgical procedures, a de Fonbrune-type microforge, a Narashige pipette grinder, and a Narashige pneumatic microinjector.
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