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1) Calculate the percentage of phenotypically mutant offspring generated by the mating cross of 2 heterozygotes for mutation, m - M/m x M/m:
a) if m is an autosomal recessive mutation?
The geneotype of these mutant is _______________?
The phenotype of the parent is ________________?
b) m is an autosomal dominant mutation?
The genotype of these mutants is ________________?
The phenotype of the parents is _________________?
2) Indicate what type of nucleic acid molecules and method is used to screen for:
a) positive transgenic founders?
b) positive transgenic expressers?
3) A transgenic founder is bred to establish a transgenic mouse line. If none of the founders offspring carry the transgene, the founder is _______________. If the
founder carries the transgene but does not express the transgene, will his offspring express the transgene?
4) Mice generated by ES cell knock-out mutations are called ________________. The knock-out mutation is only passed on to offspring if the mutation is in
_________________ cells. The offspring will be ______________ for the mutation with a genotype of ____________. Coat color is used to distinguish the F1
generations with ______________ coats and the F2 generation with ________________ coats.
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