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Nucleic Acid Biotechnology Techniques
1) Southern hybridization entails:
a) digesting _____________ molecules with ______________ _____________,
b) separating the fragments by __________________,
c) hybridizing the separating fragments to a __________________ probe,
d) and visualizing the specific fragment by __________________.
2) The three steps in Polymerase Chain Reaction (PCR):
a) ______________ double-stranded DNA molecules by _______________.
b) ______________ short oligonucleotide primers by _______________.
c) ______________ a new DNA strand using _________ _______________.
The three components crucial for carrying out a PCR reaction:
1) ______________ 2) ______________ 3) ______________
3) DNA sequencing is a technique used for determining the ____________ of _________ in a DNA molecule.
4) DNA libraries are a collection of DNA fragments. The two types of DNA libraries consist of:
a) ____________ libraries generated from digesting DNA molecules with ______ _____________.
b) ___________ libraried generated from mRNA molecules by copying the mRNA sequence into a DNA sequence using ______________
_____________.
5) Indicate all techniques (a-d) that can be used to accomplish each application listed (I-IV):
a) Southern hybridization
b) Sequencing
c) Polymerase Chain Reaction
d) Cloning
I. Amplification of DNA sequences. _______________
II. Diagnostic analysis of base mutations in DNA. __________
III. Production of a recombinant genetically engineered protein. ________
IV. Isolation of a new gene that was previously unknown. ____________
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