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Nucleic Acid Biotechnology Techniques
1) Southern hybridization entails:
a) digesting DNA molecules with restriction enzymes ,
b) separating the fragments gel electrophoresis ,
c) hybridizing the separating fragments to a radiolabelled DNA probe,
d) and visualizing the specific fragment by autoradiography .
2) The three steps in Polymerase Chain Reaction (PCR):
a) Melt double-stranded DNA molecules by heating.
b) Anneal short oligonucleotide primers by cooling.
c) Synthesize a new DNA strand using Taq
polymerase.
The three components crucial for carrying out a PCR reaction: 1) DNA
template 2) Primers 3)
Taq polymerase
3) DNA sequencing is a technique used for determining the order of
bases in a DNA molecule.
4) DNA libraries are a collection of DNA fragments. The two types of DNA libraries consist of:
a) genomic libraries generated from digesting DNA molecules with
restriction enzymes.
b) cDNA libraried generated from mRNA molecules by copying the mRNA sequence into
a DNA sequence using reverse transcriptase.
5) Indicate all techniques (a-d) that can be used to accomplish each application listed (I-IV):
a) Southern hybridization
b) Sequencing
c) Polymerase Chain Reaction
d) Cloning
I. Amplification of DNA sequences. c & d
II. Diagnostic analysis of base mutations in DNA. a &
b
III. Production of a recombinant genetically engineered protein. d
IV. Isolation of a new gene that was previously unknown. d
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