pIN4OR 8122 30 XbaI 371 NruI 408 EcoRI 456 SacI 1214 Ecl136 1214 BsrGI 1251 Sse8387 1828 NotI 1989 XmaIII 1990 NheI 2212 AvrII 2656 HindIII 2914 Bpu1102 2927 DraIII 3062 AgeI 3394 TthI 3501 EcoNI 3649 BstEII 4413 BssHII 4643 EcoRV 4680 SexAI 5091 Bst1107 5200 AccI 5200 Eco47-3 5924 BspLU11 6268 AhdI 7156 BsaI 7228 PvuI 7528 ScaI 7639 AatII 8079 7 lpp-lac PO>promoter sequence 1 391 cw N ompAompA signal peptide 392 454 cw N Reductaserat reductase coding sequence 455 2512 cw N lpp trmlipoprotein gene trx terminator 2928 3209 cw N lac Ilac I gene 3804 5027 cw N pUC ORIorigin of replication 6449 6274 ccw N Ampbeta-lactamase 7951 7088 ccw N 0 sequence TAGGCGTATCACGAGGCCCTTTGGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGCTAGAGAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGGATTCACTGGAACTCTAGATAACGAGGGCAAAAAATGAAAAAGACAGCTATCGCGATTGCAGTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGGAATTCCCGGGGATCCGACCAACATGGGGGACTCTCACGAAGACACCAGTGCCACCATGCCTGAGGCCGTGGCTGAAGAAGTGTCTCTATTCAGCACGACGGACATGGTTCTGTTTTCTCTCATCGTGGGGGTCCTGACCTACTGGTTCATCTTTAGAAAGAAGAAAGAAGAGATACCGGAGTTCAGCAAGATCCAAACAACGGCCCCACCCGTCAAAGAGAGCAGCTTCGTGGAAAAGATGAAGAAAACGGGAAGGAACATTATCGTATTCTATGGCTCCCAGACGGGAACCGCTGAGGAGTTTGCCAACCGGCTGTCCAAGGATGCCCACCGCTACGGGATGCGGGGCATGTCCGCAGACCCTGAAGAGTATGACTTGGCCGACCTGAGCAGCCTGCCTGAGATCGACAAGTCCCTGGTAGTCTTCTGCATGGCCACATACGGAGAGGGCGACCCCACGGACAATGCGCAGGACTTCTATGACTGGCTGCAGGAGACTGACGTGGACCTCACTGGGGTCAAGTTTGCTGTATTTGGTCTTGGGAACAAGACCTATGAGCACTTCAATGCCATGGGCAAGTATGTGGACCAGAGGCTGGAGCAGCTTGGCGCCCAGCGCATCTTTGAGTTGGGCCTTGGTGATGATGACGGGAACTTGGAAGAGGATTTCATCACGTGGAGGGAGCAGTTCTGGCCAGCTGTGTGCGAGTTCTTTGGGGTAGAAGCCACTGGGGAGGAGTCGAGCATTCGCCAGTATGAGCTCGTGGTCCACGAAGACATGGACGTAGCCAAGGTGTACACGGGTGAGATGGGCCGTCTGAAGAGCTACGAGAACCAGAAACCCCCCTTCGATGCTAAGAATCCATTCCTGGCTGCTGTCACCGCCAACCGGAAGCTGAACCAAGGCACTGAGCGGCATCTAATGCACCTGGAGTTGGACATCTCAGACTCCAAGATCAGGTATGAATCTGGAGATCACGTGGCTGTGTACCCAGCCAATGACTCAGCCCTGGTCAACCAGATTGGGGAGATCCTGGGAGCTGACCTGGATGTCATCATGTCTCTAAACAATCTCGATGAGGAGTCAAACAAGAAGCATCCGTTCCCCTGCCCCACCACCTACCGCACGGCCCTCACCTACTACCTGGACATCACTAACCCGCCACGCACCAATGTGCTCTACGAACTGGCACAGTACGCCTCAGAGCCCTCGGAGCAGGAGCACCTGCACAAGATGGCGTCATCCTCAGGCGAGGGCAAGGAGCTGTACCTGAGCTGGGTGGTGGAAGCCCGGAGGCACATCCTAGCCATCCTCCAAGACTACCCATCACTGCGGCCACCCATCGACCACCTGTGTGAGCTGCTGCCACGCCTGCAGGCCCGATACTACTCCATTGCCTCATCCTCCAAGGTCCACCCCAACTCCGTGCACATCTGTGCCGTGGCCGTGGAGTACGAAGCGAAGTCTGGCCGAGTGAACAAGGGGGTGGCCACTAGCTGGCTTCGGGCCAAGGAACCAGCAGGCGAGAATGGCGGCCGCGCCCTGGTACCCATGTTCGTGCGCAAATCTCAGTTCCGCTTGCCTTTCAAGTCCACCACACCTGTCATCATGGTGGGCCCCGGCACTGGGATTGCCCCTTTCATGGGCTTCATCCAGGAACGAGCTTGGCTTCGAGAGCAAGGCAAGGAGGTGGGAGAGACGCTGCTATACTATGGCTGCCGGCGCTCGGATGAGGACTATCTGTACCGTGAAGAGCTAGCCCGCTTCCACAAGGACGGTGCCCTCACGCAGCTTAATGTGGCCTTTTCCCGGGAGCAGGCCCACAAGGTCTATGTCCAGCACCTTCTGAAGAGAGACAGGGAACACCTGTGGAAGCTGATCCACGAGGGCGGTGCCCACATCTATGTGTGCGGGGATGCTCGAAATATGGCCAAAGATGTGCAAAACACATTCTATGACATTGTGGCTGAGTTCGGGCCCATGGAGCACACCCAGGCTGTGGACTATGTTAAGAAGCTGATGACCAAGGGCCGCTACTCACTAGATGTGTGGAGCTAGGAGCTACCACCCTCCCACCCCTCGCTCCCTGTAATCACCTAACTTCTGCCGACCTCCACCTCTGGTGGTTCCTGCCTGGCCTGGACACAGGGAGGCCCAGGGACTGACTCCTCCTGGCCTGAGTGGTGCCCTCCTGGGCCCCTAGGCAGAGCCCGGTCCATTGTATCAGGCAGCCCAGCCCCAGGGCACATGGCAAGAGGGACTGGACCCACCTTTGGGTGATGGGTGCCTTAGGTCCTCTGCAGATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTTGGATCCGGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGACATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTCCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCACCGGCGTTCCCGTCCGCACGCTGGAGAACAAGGCTTTAATATCCGGTGCATTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGTCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCAAAACACTCCTGTGCAGGTCAGTGTAAACATTGACCATCCGGCAATGTGAGCCAACCGGATGAAAGCTGTCCTTTTAGTTTAGCTAAGTGCAGCGGCTTTGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTTCGTCGCGTAATTCTGGCAGACGCTGGGGGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAA description High-copy pIN-reductase expression vector annotations ; Constructed by Dr. Shinyu Chang from pIN4 by digestion with ;+Xba 1 and Hind 3 and ligating Xba 1/Hind 3 reductase fragmen ;+t from pINOR (pOR262).