pIN4omp2E1/OR 9698 18 XbaI 371 BspMII 861 AatI 1783 StuI 1783 XbaI 1947 EcoRI 2032 SacI 2790 Sse8387 3404 NotI 3565 XmaIII 3566 NheI 3788 AvrII 4232 HindIII 4490 AgeI 4970 Bst1107 6776 PvuI 9104 ScaI 9215 AatII 9655 9 lpp-lac PO>promoter sequence 1 391 cw N ompAompA signal peptide 392 454 cw N hP450 2E1hP450 2E1 cDNA sequence 464 1943 cw N ompAompA signal peptide 1968 2030 cw N Reductaserat reductase coding sequence 2031 4088 cw N lpp trmlipoprotein gene trx terminator 4504 4785 cw N lac Ilac I gene 5380 6603 cw N pUC ORIorigin of replication 8025 7850 ccw N Ampbeta-lactamase 9527 8664 ccw N 0 sequence TAGGCGTATCACGAGGCCCTTTGGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTATCTAAGATGAATCCGATGGAAGCATCCTGTTTTCTCTCAATTTTTTTATCTAAAACCCAGCGTTCGATGCTTCTTTGAGCGAACGATCAAAAATAAGTGCCTTCCCATCAAAAAAATATTCTCAACATAAAAAACTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGCTAGAGAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGGATTCACTGGAACTCTAGATAACGAGGGCAAAAAATGAAAAAGACAGCTATCGCGATTGCAGTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGGAATTACCATGGCTGCCCTCGGAGTGACCGTGGCCCTGCTGGTGTGGGCGGCCTTCCTCCTGCTGGTGTCCATGTGGAGGCAGGTGCACAGCAGCTGGAATCTGCCCCCAGGCCCTTTCCCGCTTCCCATCATCGGGAACCTCTTCCAGTTGGAATTGAAGAATATTCCCAAGTCCTTCACCCGGTTGGCCCAGCGCTTCGGGCCGGTGTTCACGCTGTACGTGGGCTCGCAGCGCATGGTGGTGATGCACGGCTACAAGGCGGTGAAGGAAGCGCTGCTGGACTACAAGGACGAGTTCTCGGGCAGAGGCGACCTCCCCGCGTTCCATGCGCACAGGGACAGGGGAATCATTTTTAATAATGGACCTACCTGGAAGGACATCCGGCGGTTTTCCCTGACCACCCTCCGGAACTATGGGATGGGGAAACAGGGCAATGAGAGCCGGATCCAGAGGGAGGCCCACTTCCTGCTGGAAGCACTCAGGAAGACCCAAGGCCAGCCTTTTGACCCCACCTTCCTCATCGGCTGCGCGCCCTGCAACGTCATAGCCGACATCCTCTTCCGCAAGCATTTTGACTACAATGATGAGAAGTTTCTAAGGCTGATGTATTTGTTTAATGAGAACTTCCACCTACTCAGCACTCCCTGGCTCCAGCTTTACAATAATTTTCCCAGCTTTCTACACTACTTGCCTGGAAGCCACAGAAAAGTCATAAAAAATGTGGCTGAAGTAAAAGAGTATGTGTCTGAAAGGGTGAAGGAGCACCATCAATCTCTGGACCCCAACTGTCCCCGGGACCTCACCGACTGCCTGCTCGTGGAAATGGAGAAGGAAAAGCACAGTGCAGAGCGCTTGTACACAATGGACGGTATCACCGTGACTGTGGCCGACCTGTTCTTTGCGGGGACAGAGACCACCAGCACAACTCTGAGATATGGGCTCCTGATTCTCATGAAATACCCTGAGATCGAAGAGAAGCTCCATGAAGAAATTGACAGGGTGATTGGGCCAAGCCGAATCCCTGCCATCAAGGATAGGCAAGAGATGCCCTACATGGATGCTGTGGTGCATGAGATTCAGCGGTTCATCACCCTCGTGCCCTCCAACCTGCCCCATGAAGCAACCCGAGACACCATTTTCAGAGGATACCTCATCCCCAAGGGCACAGTCGTAGTGCCAACTCTGGACTCTGTTTTGTATGACAACCAAGAATTTCCTGATCCAGAAAAGTTTAAGCCAGAACACTTCCTGAATGAAAATGGAAAGTTCAAGTACAGTGACTATTTCAAGCCATTTTCCACAGGAAAACGAGTGTGTGCTGGAGAAGGCCTGGCTCGCATGGAGTTGTTTCTTTTGTTGTGTGCCATTTTGCAGCATTTTAATTTGAAGCCTCTCGTTGACCCAAAGGATATCGACCTCAGCCCTATACATATTGGGTTTGGCTGTATCCCACCACGTTACAAACTCTGTGTCATTCCCCGCTCATGAGTCTAGATAACGAGGGCAAAAAATGAAAAAGACAGCTATCGCGATTGCAGTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGGAATTCCCGGGGATCCGACCAACATGGGGGACTCTCACGAAGACACCAGTGCCACCATGCCTGAGGCCGTGGCTGAAGAAGTGTCTCTATTCAGCACGACGGACATGGTTCTGTTTTCTCTCATCGTGGGGGTCCTGACCTACTGGTTCATCTTTAGAAAGAAGAAAGAAGAGATACCGGAGTTCAGCAAGATCCAAACAACGGCCCCACCCGTCAAAGAGAGCAGCTTCGTGGAAAAGATGAAGAAAACGGGAAGGAACATTATCGTATTCTATGGCTCCCAGACGGGAACCGCTGAGGAGTTTGCCAACCGGCTGTCCAAGGATGCCCACCGCTACGGGATGCGGGGCATGTCCGCAGACCCTGAAGAGTATGACTTGGCCGACCTGAGCAGCCTGCCTGAGATCGACAAGTCCCTGGTAGTCTTCTGCATGGCCACATACGGAGAGGGCGACCCCACGGACAATGCGCAGGACTTCTATGACTGGCTGCAGGAGACTGACGTGGACCTCACTGGGGTCAAGTTTGCTGTATTTGGTCTTGGGAACAAGACCTATGAGCACTTCAATGCCATGGGCAAGTATGTGGACCAGAGGCTGGAGCAGCTTGGCGCCCAGCGCATCTTTGAGTTGGGCCTTGGTGATGATGACGGGAACTTGGAAGAGGATTTCATCACGTGGAGGGAGCAGTTCTGGCCAGCTGTGTGCGAGTTCTTTGGGGTAGAAGCCACTGGGGAGGAGTCGAGCATTCGCCAGTATGAGCTCGTGGTCCACGAAGACATGGACGTAGCCAAGGTGTACACGGGTGAGATGGGCCGTCTGAAGAGCTACGAGAACCAGAAACCCCCCTTCGATGCTAAGAATCCATTCCTGGCTGCTGTCACCGCCAACCGGAAGCTGAACCAAGGCACTGAGCGGCATCTAATGCACCTGGAGTTGGACATCTCAGACTCCAAGATCAGGTATGAATCTGGAGATCACGTGGCTGTGTACCCAGCCAATGACTCAGCCCTGGTCAACCAGATTGGGGAGATCCTGGGAGCTGACCTGGATGTCATCATGTCTCTAAACAATCTCGATGAGGAGTCAAACAAGAAGCATCCGTTCCCCTGCCCCACCACCTACCGCACGGCCCTCACCTACTACCTGGACATCACTAACCCGCCACGCACCAATGTGCTCTACGAACTGGCACAGTACGCCTCAGAGCCCTCGGAGCAGGAGCACCTGCACAAGATGGCGTCATCCTCAGGCGAGGGCAAGGAGCTGTACCTGAGCTGGGTGGTGGAAGCCCGGAGGCACATCCTAGCCATCCTCCAAGACTACCCATCACTGCGGCCACCCATCGACCACCTGTGTGAGCTGCTGCCACGCCTGCAGGCCCGATACTACTCCATTGCCTCATCCTCCAAGGTCCACCCCAACTCCGTGCACATCTGTGCCGTGGCCGTGGAGTACGAAGCGAAGTCTGGCCGAGTGAACAAGGGGGTGGCCACTAGCTGGCTTCGGGCCAAGGAACCAGCAGGCGAGAATGGCGGCCGCGCCCTGGTACCCATGTTCGTGCGCAAATCTCAGTTCCGCTTGCCTTTCAAGTCCACCACACCTGTCATCATGGTGGGCCCCGGCACTGGGATTGCCCCTTTCATGGGCTTCATCCAGGAACGAGCTTGGCTTCGAGAGCAAGGCAAGGAGGTGGGAGAGACGCTGCTATACTATGGCTGCCGGCGCTCGGATGAGGACTATCTGTACCGTGAAGAGCTAGCCCGCTTCCACAAGGACGGTGCCCTCACGCAGCTTAATGTGGCCTTTTCCCGGGAGCAGGCCCACAAGGTCTATGTCCAGCACCTTCTGAAGAGAGACAGGGAACACCTGTGGAAGCTGATCCACGAGGGCGGTGCCCACATCTATGTGTGCGGGGATGCTCGAAATATGGCCAAAGATGTGCAAAACACATTCTATGACATTGTGGCTGAGTTCGGGCCCATGGAGCACACCCAGGCTGTGGACTATGTTAAGAAGCTGATGACCAAGGGCCGCTACTCACTAGATGTGTGGAGCTAGGAGCTACCACCCTCCCACCCCTCGCTCCCTGTAATCACCTAACTTCTGCCGACCTCCACCTCTGGTGGTTCCTGCCTGGCCTGGACACAGGGAGGCCCAGGGACTGACTCCTCCTGGCCTGAGTGGTGCCCTCCTGGGCCCCTAGGCAGAGCCCGGTCCATTGTATCAGGCAGCCCAGCCCCAGGGCACATGGCAAGAGGGACTGGACCCACCTTTGGGTGATGGGTGCCTTAGGTCCTCTGCAGATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTTGGATCCGGCTGAGCAACGACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGACATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTCCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCACCGGCGTTCCCGTCCGCACGCTGGAGAACAAGGCTTTAATATCCGGTGCATTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGTCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCGATAGTGTTATTGGCTTCAAGGATCAACATTGCCGCAGTATCAAAACGTCGGGCAATAGCCTGAAGGTTTTTATCCCCTTCTTGCACCGTATACGTTTGATTTTGCCCAACCAGTCGGCTTCCGGTTGGTGGTAGCGGATAATCAACCGCCCAGGCAGCCTGGATGGCGCTAAAAGCGCCGATAAGCGTGAGTGTAAGCAAAGACGCGCGTTTCATTGTAAACCTCCTGTATTTGCCGGAGACTCACGCTGAAACGTCGGATGGCGCTTATGTTCACCTGAAACCAAAACACTCCTGTGCAGGTCAGTGTAAACATTGACCATCCGGCAATGTGAGCCAACCGGATGAAAGCTGTCCTTTTAGTTTAGCTAAGTGCAGCGGCTTTGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTTCGTCGCGTAATTCTGGCAGACGCTGGGGGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAA description High-copy P450/Reductase coexpression vector annotations ; Constructed by Dr. Shinyu Chang from pIN4OR by digestion wit ;+h Xba 1 and ligation of Xba 1 fragment from pINomp2E1. Resu ;+lting vector coexpresses human P450 2E1 with ompA signal pep ;+tide at N-terminus and rat P450 reductase (also with ompA si ;+gnal peptide).