SES```p    0      0tac promotertac promoter and trans. enhancer     0T7 TEXuxT7 gene 10 translational enhancer (TE)     pP450 2E1P450 3a     N5S RNA T1 T25S RNA T1 T2     Y Amp+WORKb-lactamase     ? pUC ORIorigin of replicationDXCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATATTCTGAAATGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGAATTCTGCAGTTTAACTTTAGTAAGGAGTCTAGACCATGGCTGTTCTGGGCATCACCGTCGCCCTGCTGGGGTGGATGGTCATCCTCCTGTTCATATCCGTCTGGAAGCAGATCCACAGCAGCTGGAACCTGCCCCCAGGACCTTTCCCACTGCCCATCATCGGGAATCTTCTCCAGTTGGATTTGAAGGATATTCCCAAGTCCTTTGGCAGGCTGGCAGAGCGCTTTGGGCCGGTGTTCACTGTGTACCTGGGCTCCAGGCGTGTTGTGGTTCTGCACGGCTACAAGGCGGTGAGGGAGATGCTGTTGAACCACAAGAACGAGTTCTCTGGGCGTGGCGAGATCCCTGCTTTCCGGGAGTTTAAGGACAAGGGGATCATTTTCAACAATGGACCCACCTGGAAGGACACTCGGCGGTTCTCCCTGACCACCCTCCGGGACTATGGGATGGGGAAACAGGGCAACGAGGACCGGATCCAGAAGGAGGCCCACTTCCTGCTGGAGGAGCTCAGGAAGACCCAGGGCCAGCCCTTCGACCCCACCTTTGTCATCGGCTGCACACCCTTCAACGTCATCGCCAAAATCCTCTTCAATGACCGCTTTGACTATAAGGACAAGCAGGCTCTGAGGCTGATGAGTTTGTTCAACGAGAACTTCTACCTGCTCAGTACTCCTTGGCTGCAGGTTTACAATAATTTTTCAAACTATCTACAGTACATGCCTGGAAGTCACAGGAAAGTAATAAAAAATGTGTCTGAAATAAAAGAGTACACACTCGCAAGAGTGAAGGAGCACCACAAGTCGCTGGACCCCAGCTGCCCCCGGGACTTCATTGACAGCCTGCTCATAGAAATGGAGAAGGACAAACACAGCACGGAGCCCCTGTACACGCTGGAAAACATTGCTGTGACTGTGGCGGACATGTTCTTTGCGGGCACGGAGACCACCAGCACCACGCTGCGATATGGGCTCCTGATCCTGCTGAAGCACCCCGAGATCGAAGAGAAACTTCATGAAGAAATCGACAGGGTGATTGGGCCGAGCCGAATGCCTTCTGTCAGGGACAGGGTGCAGATGCCCTACATGGACGCTGTGGTACATGAGATTCAGCGATTCATCGATCTCGTGCCCTCCAATCTGCCGCACGAAGCCACACGGGACACCACCTTCCAAGGATACGTCATCCCCAAGGGCACTGTTGTAATCCCGACTCTGGACTCCCTTTTGTATGACAAGCAAGAATTCCCTGATCCCGAGAAGTTCAAACCAGAGCACTTTCTGAATGAGGAGGGGAAGTTCAAGTATAGCGACTACTTCAAGCCGTTTTCCGCAGGAAAACGCGTGTGTGTTGGAGAAGGCCTGGCTCGCATGGAGTTGTTTCTGCTCCTGTCTGCCATTCTGCAGCATTTTAACCTCAAGCCTCTCGTTGACCCAGAGGACATTGACCTTCGCAATATTACGGTGGGCTTTGGCCGTGTCCCACCACGCTACAAACTCTGTGTCATTCCCCGCTCGTAAACCCAAGGGCAGCACCCAGAGGCCACTCTTCTCCTCGACCTGCAGCCCAAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATGCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCGAGCTCGCCCGGGGATCGATCCGGAGCTTATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCATCGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAGGCGCACTCC -EcoRIPstIXbaINcoIBsrGIMluIHindIII . _  $Sxexxxx @xxxpJL2/2E1(rabt)2Rabbit P450 2E1 expression vectorEcoRIdentical to pJL3a, but with use of pJL2 vector [Bam HI-Bam HI religation to remove unneeded restriction sites]. ?