LOCUS pIN4omp2E1 9698 bp DNA CIRCULAR SYN 01-FEB-1996 DEFINITION High-copy P450/Reductase coexpression vector ACCESSION pIN4omp2E1 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9698) AUTHORS Self JOURNAL Unpublished. FEATURES Location/Qualifiers CDS 392..454 /gene="ompA" /product="ompA signal peptide" CDS 464..1943 /gene="hP450 2E1" /product="hP450 2E1 cDNA sequence" CDS 1968..2030 /gene="ompA" /product="ompA signal peptide" CDS 2031..4088 /gene="Reductase" /product="rat reductase coding sequence" CDS 5380..6603 /gene="lac I" /product="lac I gene" CDS complement (8664..9527) /gene="Amp" /product="beta-lactamase" BASE COUNT 2339 a 2598 c 2524 g 2237 t ORIGIN 1 TAGGCGTATC ACGAGGCCCT TTGGATAACC AGAAGCAATA AAAAATCAAA TCGGATTTCA 61 CTATATAATC TCACTTTATC TAAGATGAAT CCGATGGAAG CATCCTGTTT TCTCTCAATT 121 TTTTTATCTA AAACCCAGCG TTCGATGCTT CTTTGAGCGA ACGATCAAAA ATAAGTGCCT 181 TCCCATCAAA AAAATATTCT CAACATAAAA AACTTTGTGT AATACTTGTA ACGCTACATG 241 GAGATTAACT CAATCTAGCT AGAGAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT 301 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGGAT 361 TCACTGGAAC TCTAGATAAC GAGGGCAAAA AATGAAAAAG ACAGCTATCG CGATTGCAGT 421 GGCACTGGCT GGTTTCGCTA CCGTAGCGCA GGCCGGAATT ACCATGGCTG CCCTCGGAGT 481 GACCGTGGCC CTGCTGGTGT GGGCGGCCTT CCTCCTGCTG GTGTCCATGT GGAGGCAGGT 541 GCACAGCAGC TGGAATCTGC CCCCAGGCCC TTTCCCGCTT CCCATCATCG GGAACCTCTT 601 CCAGTTGGAA TTGAAGAATA TTCCCAAGTC CTTCACCCGG TTGGCCCAGC GCTTCGGGCC 661 GGTGTTCACG CTGTACGTGG GCTCGCAGCG CATGGTGGTG ATGCACGGCT ACAAGGCGGT 721 GAAGGAAGCG CTGCTGGACT ACAAGGACGA GTTCTCGGGC AGAGGCGACC TCCCCGCGTT 781 CCATGCGCAC AGGGACAGGG GAATCATTTT TAATAATGGA CCTACCTGGA AGGACATCCG 841 GCGGTTTTCC CTGACCACCC TCCGGAACTA TGGGATGGGG AAACAGGGCA ATGAGAGCCG 901 GATCCAGAGG GAGGCCCACT TCCTGCTGGA AGCACTCAGG AAGACCCAAG GCCAGCCTTT 961 TGACCCCACC TTCCTCATCG GCTGCGCGCC CTGCAACGTC ATAGCCGACA TCCTCTTCCG 1021 CAAGCATTTT GACTACAATG ATGAGAAGTT TCTAAGGCTG ATGTATTTGT TTAATGAGAA 1081 CTTCCACCTA CTCAGCACTC CCTGGCTCCA GCTTTACAAT AATTTTCCCA GCTTTCTACA 1141 CTACTTGCCT GGAAGCCACA GAAAAGTCAT AAAAAATGTG GCTGAAGTAA AAGAGTATGT 1201 GTCTGAAAGG GTGAAGGAGC ACCATCAATC TCTGGACCCC AACTGTCCCC GGGACCTCAC 1261 CGACTGCCTG CTCGTGGAAA TGGAGAAGGA AAAGCACAGT GCAGAGCGCT TGTACACAAT 1321 GGACGGTATC ACCGTGACTG TGGCCGACCT GTTCTTTGCG GGGACAGAGA CCACCAGCAC 1381 AACTCTGAGA TATGGGCTCC TGATTCTCAT GAAATACCCT GAGATCGAAG AGAAGCTCCA 1441 TGAAGAAATT GACAGGGTGA TTGGGCCAAG CCGAATCCCT GCCATCAAGG ATAGGCAAGA 1501 GATGCCCTAC ATGGATGCTG TGGTGCATGA GATTCAGCGG TTCATCACCC TCGTGCCCTC 1561 CAACCTGCCC CATGAAGCAA CCCGAGACAC CATTTTCAGA GGATACCTCA TCCCCAAGGG 1621 CACAGTCGTA GTGCCAACTC TGGACTCTGT TTTGTATGAC AACCAAGAAT TTCCTGATCC 1681 AGAAAAGTTT AAGCCAGAAC ACTTCCTGAA TGAAAATGGA AAGTTCAAGT ACAGTGACTA 1741 TTTCAAGCCA TTTTCCACAG GAAAACGAGT GTGTGCTGGA GAAGGCCTGG CTCGCATGGA 1801 GTTGTTTCTT TTGTTGTGTG CCATTTTGCA GCATTTTAAT TTGAAGCCTC TCGTTGACCC 1861 AAAGGATATC GACCTCAGCC CTATACATAT TGGGTTTGGC TGTATCCCAC CACGTTACAA 1921 ACTCTGTGTC ATTCCCCGCT CATGAGTCTA GATAACGAGG GCAAAAAATG AAAAAGACAG 1981 CTATCGCGAT TGCAGTGGCA CTGGCTGGTT TCGCTACCGT AGCGCAGGCC GGAATTCCCG 2041 GGGATCCGAC CAACATGGGG GACTCTCACG AAGACACCAG TGCCACCATG CCTGAGGCCG 2101 TGGCTGAAGA AGTGTCTCTA TTCAGCACGA CGGACATGGT TCTGTTTTCT CTCATCGTGG 2161 GGGTCCTGAC CTACTGGTTC ATCTTTAGAA AGAAGAAAGA AGAGATACCG GAGTTCAGCA 2221 AGATCCAAAC AACGGCCCCA CCCGTCAAAG AGAGCAGCTT CGTGGAAAAG ATGAAGAAAA 2281 CGGGAAGGAA CATTATCGTA TTCTATGGCT CCCAGACGGG AACCGCTGAG GAGTTTGCCA 2341 ACCGGCTGTC CAAGGATGCC CACCGCTACG GGATGCGGGG CATGTCCGCA GACCCTGAAG 2401 AGTATGACTT GGCCGACCTG AGCAGCCTGC CTGAGATCGA CAAGTCCCTG GTAGTCTTCT 2461 GCATGGCCAC ATACGGAGAG GGCGACCCCA CGGACAATGC GCAGGACTTC TATGACTGGC 2521 TGCAGGAGAC TGACGTGGAC CTCACTGGGG TCAAGTTTGC TGTATTTGGT CTTGGGAACA 2581 AGACCTATGA GCACTTCAAT GCCATGGGCA AGTATGTGGA CCAGAGGCTG GAGCAGCTTG 2641 GCGCCCAGCG CATCTTTGAG TTGGGCCTTG GTGATGATGA CGGGAACTTG GAAGAGGATT 2701 TCATCACGTG GAGGGAGCAG TTCTGGCCAG CTGTGTGCGA GTTCTTTGGG GTAGAAGCCA 2761 CTGGGGAGGA GTCGAGCATT CGCCAGTATG AGCTCGTGGT CCACGAAGAC ATGGACGTAG 2821 CCAAGGTGTA CACGGGTGAG ATGGGCCGTC TGAAGAGCTA CGAGAACCAG AAACCCCCCT 2881 TCGATGCTAA GAATCCATTC CTGGCTGCTG TCACCGCCAA CCGGAAGCTG AACCAAGGCA 2941 CTGAGCGGCA TCTAATGCAC CTGGAGTTGG ACATCTCAGA CTCCAAGATC AGGTATGAAT 3001 CTGGAGATCA CGTGGCTGTG TACCCAGCCA ATGACTCAGC CCTGGTCAAC CAGATTGGGG 3061 AGATCCTGGG AGCTGACCTG GATGTCATCA TGTCTCTAAA CAATCTCGAT GAGGAGTCAA 3121 ACAAGAAGCA TCCGTTCCCC TGCCCCACCA CCTACCGCAC GGCCCTCACC TACTACCTGG 3181 ACATCACTAA CCCGCCACGC ACCAATGTGC TCTACGAACT GGCACAGTAC GCCTCAGAGC 3241 CCTCGGAGCA GGAGCACCTG CACAAGATGG CGTCATCCTC AGGCGAGGGC AAGGAGCTGT 3301 ACCTGAGCTG GGTGGTGGAA GCCCGGAGGC ACATCCTAGC CATCCTCCAA GACTACCCAT 3361 CACTGCGGCC ACCCATCGAC CACCTGTGTG AGCTGCTGCC ACGCCTGCAG GCCCGATACT 3421 ACTCCATTGC CTCATCCTCC AAGGTCCACC CCAACTCCGT GCACATCTGT GCCGTGGCCG 3481 TGGAGTACGA AGCGAAGTCT GGCCGAGTGA ACAAGGGGGT GGCCACTAGC TGGCTTCGGG 3541 CCAAGGAACC AGCAGGCGAG AATGGCGGCC GCGCCCTGGT ACCCATGTTC GTGCGCAAAT 3601 CTCAGTTCCG CTTGCCTTTC AAGTCCACCA CACCTGTCAT CATGGTGGGC CCCGGCACTG 3661 GGATTGCCCC TTTCATGGGC TTCATCCAGG AACGAGCTTG GCTTCGAGAG CAAGGCAAGG 3721 AGGTGGGAGA GACGCTGCTA TACTATGGCT GCCGGCGCTC GGATGAGGAC TATCTGTACC 3781 GTGAAGAGCT AGCCCGCTTC CACAAGGACG GTGCCCTCAC GCAGCTTAAT GTGGCCTTTT 3841 CCCGGGAGCA GGCCCACAAG GTCTATGTCC AGCACCTTCT GAAGAGAGAC AGGGAACACC 3901 TGTGGAAGCT GATCCACGAG GGCGGTGCCC ACATCTATGT GTGCGGGGAT GCTCGAAATA 3961 TGGCCAAAGA TGTGCAAAAC ACATTCTATG ACATTGTGGC TGAGTTCGGG CCCATGGAGC 4021 ACACCCAGGC TGTGGACTAT GTTAAGAAGC TGATGACCAA GGGCCGCTAC TCACTAGATG 4081 TGTGGAGCTA GGAGCTACCA CCCTCCCACC CCTCGCTCCC TGTAATCACC TAACTTCTGC 4141 CGACCTCCAC CTCTGGTGGT TCCTGCCTGG CCTGGACACA GGGAGGCCCA GGGACTGACT 4201 CCTCCTGGCC TGAGTGGTGC CCTCCTGGGC CCCTAGGCAG AGCCCGGTCC ATTGTATCAG 4261 GCAGCCCAGC CCCAGGGCAC ATGGCAAGAG GGACTGGACC CACCTTTGGG TGATGGGTGC 4321 CTTAGGTCCT CTGCAGATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC 4381 CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC 4441 ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTGGATC 4501 CGGCTGAGCA ACGACGTGAA CGCAATGCGT TCCGACGTTC AGGCTGCTAA AGATGACGCA 4561 GCTCGTGCTA ACCAGCGTCT GGACAACATG GCTACTAAAT ACCGCAAGTA ATAGTACCTG 4621 TGAAGTGAAA AATGGCGCAC ATTGTGCGAC ATTTTTTTTG TCTGCCGTTT ACCGCTACTG 4681 CGTCACGCGT AACATATTCC CTTGCTCTGG TTCACCATTC TGCGCTGACT CTACTGAAGG 4741 CGCATTGCTG GCTGCGGGAG TTGCTCCACT GCTCACCGAA ACCGGATACC CTGCCCGACG 4801 ATACAACGCT TTATCGACTA ACTTCTGATC TACAGCCTTA TTGTCTTTAA ATTGCGTAAA 4861 GCCTGCTGGC AGTGTGTATG GCATTGTCTG AACGTTCTGC TGTTCTCCTG CCGATAGTGG 4921 TCGATGTACT TCAACATAAC GCATCCCGTT AGGCTCCACG GAATATTTCA CCGGTTCGTT 4981 GATCACTTTC ACCGGCGTTC CCGTCCGCAC GCTGGAGAAC AAGGCTTTAA TATCCGGTGC 5041 ATTCATGCGA ATACACCCTG AACTGACGCG CAAACCGACG CTGTCCGGCG CACTGGTACC 5101 ATGAATGAGG TATTCGCCAT TACCATGCGC GAGGCGCAGT GCGTAACGTC CTAGCGGGTT 5161 ATTTGGTCCG GCAGGAACGA CTGGCGGTAA TTTAATGCCA CGCTCCAGCG AACGCTGACG 5221 AATGCCTGCC GTAGGCGTCC AGGTTGGGTT AGGGATTTTC TGCCCAACAC GCGTTTCCAT 5281 CACCGGCGTT TCCAGCCCCT GCAATCCAAT ACCTATTGGA TAAACCTGCA CAATATTTTC 5341 TCCCGGCGGA TAATAATAAA GGCGCAGCTC TGCAAGGTTG ACACCATCGA ATGGCGCAAA 5401 ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT GGTGAATGTG 5461 AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA GACCGTTTCC 5521 CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG 5581 ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG 5641 TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG 5701 GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTAGAACGA 5761 AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG 5821 CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT 5881 AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG TATTATTTTC 5941 TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG TCACCAGCAA 6001 ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT GGCTGGCTGG 6061 CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG AACGGGAAGG CGACTGGAGT 6121 GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG AGGGCATCGT TCCCACTGCG 6181 ATGCTGGTTG CCAACGATCA GATGGCGCTG GGCGCAATGC GCGCCATTAC CGAGTCCGGG 6241 CTGCGCGTTG GTGCGGATAT CTCGGTAGTG GGATACGACG ATACCGAAGA CAGCTCATGT 6301 TATATCCCGC CGTCAACCAC CATCAAACAG GATTTTCGCC TGCTGGGGCA AACCAGCGTG 6361 GACCGCTTGC TGCAACTCTC TCAGGGCCAG GCGGTGAAGG GCAATCAGCT GTTGCCCGTC 6421 TCACTGGTGA AAAGAAAAAC CACCCTGGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG 6481 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA 6541 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT 6601 GCTAACGATA ATCCCCTGAC GCGGTGCATC AGGTAATAAC AGTTGTGAAG GAATAGTTAT 6661 CGTCGTACCA GGTTTTGGCA CCGGGGCGAT AGTGTTATTG GCTTCAAGGA TCAACATTGC 6721 CGCAGTATCA AAACGTCGGG CAATAGCCTG AAGGTTTTTA TCCCCTTCTT GCACCGTATA 6781 CGTTTGATTT TGCCCAACCA GTCGGCTTCC GGTTGGTGGT AGCGGATAAT CAACCGCCCA 6841 GGCAGCCTGG ATGGCGCTAA AAGCGCCGAT AAGCGTGAGT GTAAGCAAAG ACGCGCGTTT 6901 CATTGTAAAC CTCCTGTATT TGCCGGAGAC TCACGCTGAA ACGTCGGATG GCGCTTATGT 6961 TCACCTGAAA CCAAAACACT CCTGTGCAGG TCAGTGTAAA CATTGACCAT CCGGCAATGT 7021 GAGCCAACCG GATGAAAGCT GTCCTTTTAG TTTAGCTAAG TGCAGCGGCT TTGGCGCGAA 7081 TTGCGCGAAT CATCGCTTCC AGACCTTGTG AACGAGATGG GGTGAGATGT TGGGTGAGCG 7141 CCATTTTTTC AAACCACGGA CGCACATCGA AATTGACAAT ATCCTGCGGC GTCATCTGAT 7201 CGTAGAGAAT AAAGACGACC GCAATAAGCC CTTTCACAAT CGCCGCATCG CTGTCGCCCT 7261 GTAATTCAAT AATTCCCTGG GCATTCTGGC GCATGACAAT CCACACCTGA CTCTGACAGC 7321 CCTGAATGCT ATTTTGTGGA CTTCTGTCTT CGTCGCGTAA TTCTGGCAGA CGCTGGGGGA 7381 CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT 7441 ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA 7501 GCGCTCTGGG TCATTTTCGG CGAGGACCGC TTTCGCTGGA GCGCGACGAT GATCGGCCTG 7561 TCGCTTGCGG TATTCGGAAT CTTGCACGCC CTCGCTCAAG CCTTCGTCAC TGGTCCCGCC 7621 ACCAAACGTT TCGGCGAGAA GCAGGCCATT ATCGCCGGCA TGGCGGCCCT GCATTAATGA 7681 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 7741 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 7801 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 7861 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 7921 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 7981 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 8041 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 8101 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 8161 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 8221 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 8281 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 8341 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 8401 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 8461 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 8521 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 8581 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 8641 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 8701 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 8761 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG 8821 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 8881 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 8941 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 9001 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 9061 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 9121 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 9181 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 9241 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA 9301 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 9361 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 9421 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 9481 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 9541 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 9601 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC 9661 TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAA //