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PLASMIDS

The following plasmids are available by written or email request:

Bacterial Expression Vectors

Name Description Data
pJL2 High copy vector derived from pKK223-3; contains the T7 gene 10 translational enhancer upstream of the translation start site.  Superseded by pJL3. Map Seq. File Ref. & notes
pJL3 Identical to pJL2, with the removal of unnecessary restriction sites. Map Seq File Ref. & notes
pINIIIompA3 Transiently attaches the E. coli outer membrane protein A signal peptide to N-terminus of cloned protein to facilitate membrane incorporation or secretion.   Map Seq File Ref. & notes
pIN4 High copy version of pINIIIompA3 Map Seq File Ref. & notes

 

P450 2E1 Expression Vectors

Name Description Data
pJL2/2E1rabbit

Full-length rabbit P450 2E1 cDNA in pJL2 vector (similar to pJL3).

Map Seq File Ref. & notes
pJL2/2E1human Full-length human P450 2E1 cDNA in pJL2 vector.   Map Seq File Ref. & notes
pIN3/2E1human Full-length human P450 2E1 cDNA in pINIIIompA3 vector. Map Seq File Ref. & notes
pIN4/2E1human Full-length human P450 2E1 cDNA in high-copy pIN4 vector. Map Seq File Ref. & notes

 

P450 Reductase Expression Vectors

Name Description Data
pIN3/OR

Full-length rat NADPH-cytochrome P450 reductase cDNA in pINIIIompA3 vector.

Map Seq File Ref. & notes
pIN4/OR Full-length rat NADPH-cytochrome P450 reductase cDNA in high-copy pIN4 vector. Map Seq File Ref. & notes

 

P450 2E1/Reductase Coexpression Vectors

Name Description Data
pJL2/2E1/OR

Human P450 2E1 cDNA upstream of rat P450 reductase cDNA in pJL2 vector.

Map Seq File Ref. & notes
pIN3/2E1/OR Human P450 2E1 cDNA upstream of rat P450 reductase cDNA in pINIIIompA3 vector.  Used in our recombinant CYP2E1 Ames assay (pIN3ER). Map Seq File Ref. & notes
pIN4/2E1/OR Human P450 2E1 cDNA upstream of rat P450 reductase cDNA in high-copy pIN4 vector. Map Seq File Ref. & notes

 

P450 2E1/Reductase/Cytochrome b5 Coexpression Vector

Name Description Data
pIN3/b5/2E1/OR

Full-length human cytochrome b5 cDNA upstream of human P450 2E1 and rat P450 reductase cDNAs in pINIIIompA3 vector.  Used in our recombinant CYP2E1 Ames assay (pIN3b5ER).

Map Seq File Ref. & notes

 

Squalene Monooxygenase Expression Vector

Name Description Data
pTYB4SE110

Human squalene monooxygenase cDNA lacking the first 110 amino acids in pTYB4 vector (New England Biolabs).

Map Seq File Ref. & notes

 

 

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Comments to Todd D. Porter, Pharmaceutical Sciences, University of Kentucky College of Pharmacy, Lexington, KY 40536-0082.  
Phone 859 257-1137; FAX 859 257-7564
Last Modified: November 06, 2001
Copyright 2000, University of Kentucky Chandler Medical Center