Jay Van Doorn's study focuses on the role of sulfate groups in inhibition, using shRNA technology to knockdown the carbohydrate sulfotransferase (CHST) enzyme responsible for specific CSPG sulfation patterns. These mutant CSPGs will be used in tissue culture assays to examine neurite outgrowth. An understanding of the molecular mechanisms by which CSPG inhibit regeneration may lead to potential therapies for the treatment of SCI. (Support KSCHIRT #10-11A). The mentor will be Dr. Diane Snow in the Department of Anatomy and Neurobiology.