454 GS FLX+

The GS FLX+ offers high-throughput sequencing (105 to 106 reads per run) but its advantage lies in the ability to generate reads of ≥800 bases.  It is particularly suitable for:

  • Microbial genome sequencing
  • RNAseq (gene discovery in novel genomes, full-length transcript assembly, single nucleotide polymorphism detection, insertion-deletion and splice-variant discovery)
  • Deep amplicon sequencing (mutation detection, population studies)
  • CHIP-seq

AGTC can perform all sequencing steps starting from library preparation to sequence acquisition.  If desired, we can also provide clients with recommended library construction protocols so they can make libraries in their own laboratory.  AGTC offers the following library preparation services:

  • Genomic shotgun (also ChIPseq and bisulfite-treated DNA)
  • Genomic paired-end
  • cDNA
  • normalized cDNA
  • note: All libraries, except paired end libraries can be barcoded to allow multiplexing on a single plate/field.

Standard bioinformatic services:  Each 454 GS FLX+ run comes with standard bioinformatic services as follows:

  • Genome sequencing:

         Genome assembly and/or alignment to a reference genome

  • RNAseq

         Transcript assembly and/or alignment to a reference genome

  • CHIPseq

         Alignment of reads to a reference genome

  • Barcoded libraries

         Complimentary deconvolution of multiplexed samples

Contact AGTC staff for additional information


454 GS FLX+ Template Preparation Instructions