The Illumina MiSeq machine produces between 15 million to 20 million paired end reads in ~48 h hours (24 h for single-end reads). With read lengths of up to 300 bp, the MiSeq is suitable for:
Microbial genome sequencing
AGTC can perform all sequencing steps starting from library preparation to sequence acquisition. If desired, we can also provide clients with recommended library preparation protocols so they can construct libraries in their own laboratory. AGTC offers the following library preparation services:
Genomic shotgun (up to 8 bacterial genomes per flowcell)
Genomic paired-end sequencing
Bisulfite-treated DNA libraries
PCR free library preparation (improves sequence representation for better genome assemblies
note: All libraries can be barcoded to allow multiplexing in a single flowcell.
Standard bioinformatic services: Each MiSeq run comes with standard bioinformatic services as follows:
Genome assembly and/or alignment to a reference genome
Transcript assembly and/or alignment to a reference genome
Alignment of reads to a reference genome
Complimentary deconvolution of multiplexed samples
Contact AGTC staff for additional information
Template preparation instructions: Please contact Jenny Webb in the Laboratory.