Illumina MiSeq

The Illumina MiSeq machine produces between 15 million to 20 million paired end reads in ~48 hours (24 h for single-end reads). With read lengths of up to 300 bp, the MiSeq is suitable for:

  • Microbial genome sequencing (DNA submitted must be purified from a single colony)
  • Targeted resequencing
  • Amplicon sequencing
  • cDNA sequencing
  • Transcriptome analysis
  • CHIP-seq
  • microRNA sequencing

AGTC can perform all sequencing steps starting from library preparation to sequence acquisition. If desired, we can also provide clients with recommended library preparation protocols so they can construct libraries in their own laboratory. AGTC offers the following library preparation services:

  • Genomic shotgun (up to 8 bacterial genomes per flowcell)
  • Genomic paired-end sequencing
  • CHIP-Seq libraries
  • Bisulfite-treated DNA libraries
  • PCR free library preparation (improves sequence representation for better genome assemblies)
  • cDNA
  • normalized cDNA

note: All libraries can be barcoded to allow multiplexing in a single flowcell.

Standard bioinformatic services: Each MiSeq run comes with standard bioinformatic services as follows:

  • Genome sequencing:
                Genome assembly and/or alignment to a reference genome
  • RNAseq
                Transcript assembly and/or alignment to a reference genome
  • CHIPseq
                Alignment of reads to a reference genome
  • Barcoded libraries
                Complimentary deconvolution of multiplexed samples

Contact AGTC staff for additional information

Rates

Template preparation instructions: Please contact Jenny Webb in the Laboratory.