Illumina MiSeq

The Illumina MiSeq machine produces between 15 million to 20 million paired end reads in ~48 h hours (24 h for single-end reads). With read lengths of up to 300 bp, the MiSeq is suitable for:

Microbial genome sequencing

Targeted resequencing

Amplicon sequencing

eDNA sequencing

Transcriptome analysis

CHIP-seq

microRNA sequencing

AGTC can perform all sequencing steps starting from library preparation to sequence acquisition. If desired, we can also provide clients with recommended library preparation protocols so they can construct libraries in their own laboratory. AGTC offers the following library preparation services:

Genomic shotgun (up to 8 bacterial genomes per flowcell)

Genomic paired-end sequencing

CHIP-Seq libraries

Bisulfite-treated DNA libraries

PCR free library preparation (improves sequence representation for better genome assemblies

cDNA

normalized cDNA

note: All libraries can be barcoded to allow multiplexing in a single flowcell.

Standard bioinformatic services: Each MiSeq run comes with standard bioinformatic services as follows:

Genome sequencing:

            Genome assembly and/or alignment to a reference genome

RNAseq

            Transcript assembly and/or alignment to a reference genome

CHIPseq

            Alignment of reads to a reference genome

Barcoded libraries

            Complimentary deconvolution of multiplexed samples

Contact AGTC staff for additional information

Rates

Template preparation instructions: Please contact Jenny Webb in the Laboratory.