Fragment Analysis Instructions

Fragment Analysis on the ABI3730

 

Container Name must be alphanumeric only with no spaces or characters

Plate ID should be same as Container Name

Sample name must be alphanumeric only, with no spaces or characters.  AGTC recommends using a lower case “x” in lieu of spaces

Size Std – list size standard to be use for each sample

User Defined 1 - should contain the name of the DYE SET (eg DS02)

User Defined 2 and 3 – can be used to provide additional information to assist the User with downstream analyses (alphanumerics only)

Instrument Protocol 1 - for new customers, AGTC will fill in this field based on the chosen dye set and matrix.  Experienced customers who wish to try modified protocol settings can supply protocol information in this field

  1. Clients should be registered with AGTC and have a “Lab Name”  

  2. The Dye Sets compatible with the ABI 3730 are as follows:

     

         DS02                            DS31                                       DS33

         DS30                            DS32                                       DS40

    Please see ABI publication 107MI108-01 for available dye sets and size standards

  3. PCR should be performed in MicroAmp semi-skirted plates (ABI PNN8010560).

  4. Container should be named:  FragAnaly_LabName_Date (MMDDYYYY)

  5. Client will perform PCR and dilute reactions before sending to AGTC (typically 1:15 with Hi-Di formamide: ABI PN 4311320)

  6. If client is on campus, then the size standard can be added (0.5-1ul) and sample denatured at 95C for 2 min, snap-cooled and delivered promptly to AGTC.

  7. If client is sending samples through the mail, AGTC recommends sending the diluted reactions in a plate (or plates), along with a separate tube of size standard.  AGTC will add the standard and denature immediately prior to loading on the machine

    (note: plates should be well sealed with foil or plastic adhesive)

  8. The following is a blank Sample template that should be filled out for each plate