UK-AGTC Rates

Sanger Sequencing
Run-only: client-prepared, client-purified sequencing reactions (1-13 samples) $3.45/sample
Run-only:  client-prepared, client-purified sequencing reactions (13-95 samples) $44.65 (flat rate)
React & run: AGTC performs sequencing reactions, purification and run (1-15 samples) $9.35/sample
React & run: AGTC performs sequencing reactions, purification and run (16-94 samples) $146.85 (flat rate)
High-throughput plasmid prep, react & run + QA: Sequencing of 760 (8x94) DNA templates with QA $1,158.36 (flat rate)
High-throughput plasmid prep, react & run, no QA: Sequencing of 760 (8x94) DNA templates no QA $858.08 (flat rate)
High-throughput cosmid/fosmid prep, react & run + QA: Sequencing of 760 (8x94) DNA templates with QA $1,338.99
High-throughput cosmid/fosmid prep, react & run, no QA: Sequencing of 760 (8x94) DNA templates with QA      $1,237.23 (flat rate)
Sequence reaction cleanup, ethanol precipitation (1 – 4 samples)  $0.73/sample
Sequence reaction cleanup, ethanol precipitation (5 – 96 samples) $2.37 (flat rate)
Sequence reaction cleanup, Life Technologies BigDye Xterminator (removes dye front) $1.63/sample
PCR product purification: magnetic bead prep (96-well plate – can be prorated) $110.62/plate
PCR product purification: enzymatic cleanup (96-well plate – can be prorated) $94.41/plate
Additional primers: (if AGTC performs reactions and multiple primers are to be used in a single plate) $6.20/ additional primer

 

Ion TorrentTM
Shotgun library preparation $348.51
Template preparation for one 314 or 316 chip $292.26
Template preparation for one 318 chip $533.59
Sequencing of one 314 chip $309.89 
Sequencing of one 316 chip $569.46
Sequencing of one 318 chip $827.44

 

454 Sequencing
Shotgun library preparation $844.61
GS Titanium XL run - standard or extended read lengths (Note: user can request ½, ¼ or 1/8, etc. plate to reduce costs.  Charge will be pro-rated accordingly) $4088.62/plate
Large volume emulsion PCR (prorated if run uses less than a whole plate) $1703.48/plate
cDNA DNA library construction with directional adaptors $1803.14
Paired end library 3 kb $1672.54
Paired end library 8 kb $1742.49
Paired end library 20 kb $1759.69

 

Fragment Analysis
Fragment Analysis on the ABI 3730 (client performs reactions) $48.36/ 96-well plate

 

Other AGTC Services
Robotic gridding of nylon membranes $770.44/ two plates 
Programming of robot by AGTC staff $125.60/h
Usage of AGTC equipment by staff per client request OR by client with staff support $7.54/h;
Bioanalyzer chip (standard, 1-11 samples) $34.26
Bioanalyzer chip (high sensitivity, 1-11 samples) $76.23
DNA quantification with PicoGreen (1-24 samples) $23.98
Robotic replication of 96- or 384-well plates (2 copies of 8 x 96-/384-well plates) $105.18/ 16 plates
Load and run TLDA cards on ABI HT7900 RT-PCR machine $49.91/card
Client use of Hydroshear $21.66/h
Client use of a single HiGro incubator pod (6 deep well plates per pod) $16.43/h 
Client use of thermal cycler $6.66/h 
Client use of non-robotic equipment $3.33/h 
Client use of GenoGrinder (grinding of one pair of plates constitutes a run) $18.35/run 

 

MiSeq Services
Schedule 38 - MiSeq run (2X250 flowcell) $1,317.74
Schedule 39A - Nextera Library Prep $321.09
Schedule 39B - Nextera XT Library Prep $325.41
Schedule 40A - TruSeq Library Prep $316.76
Schedule 40B - TruSeq PCR free DNA Prep $287.76
Schedule 41A - TruSeq RNA Prep $343.78
Schedule 41B - Alternative RNA Library Prep $417.05
Schedule 44 - QPCR for NGS Libraries $27.38

 

Bioinformatic Analysis 

Services are billed at $100/h cpu time