UK-AGTC Rates 

Sanger Sequencing
Run-only: client-prepared, client-purified sequencing reactions (1-12 samples) $3.46/sample
Run-only:  client-prepared, client-purified sequencing reactions (13-95 samples) $44.23 (flat rate)
React & run: AGTC performs sequencing reactions, purification and run (1-15 samples) $9.60/sample
React & run: AGTC performs sequencing reactions, purification and run (16-94 samples) $146.61 (flat rate)
Sequence reaction cleanup, ethanol precipitation (1 – 4 samples)  $0.79/sample
Sequence reaction cleanup, ethanol precipitation (5 – 96 samples) $2.65 (flat rate)
Sequence reaction cleanup, Life Technologies BigDye Xterminator (removes dye front) $3.04/sample
PCR product purification: magnetic bead prep (96-well plate – can be prorated) $116.53/plate
PCR product purification: enzymatic cleanup (per sample) $2.03/sample

 


Ion TorrentTM
Shotgun library preparation $366.31
Template preparation for one 314 or 316 chip $284.86
Sequencing of one 314 chip $318.69 
Sequencing of one 316 chip $572.93

 


454 Sequencing
Shotgun library preparation $810.87
GS Titanium XL run - standard or extended read lengths (Note: user can request ½, ¼ or 1/8, etc. plate to reduce costs.  Charge will be pro-rated accordingly) $4109.95/plate
Large volume emulsion PCR (prorated if run uses less than a whole plate) $1705.85/plate
cDNA DNA library construction with directional adaptors $1978.81
Paired end library 3 kb $1744.13
Paired end library 8 kb $1830.86

 


Fragment Analysis
Fragment Analysis on the ABI 3730 (client performs reactions) $50.01/ 96-well plate

 


Other AGTC Services
Robotic gridding of nylon membranes $472.07/ two plates 
Programming of robot by AGTC staff $211.03/h
Usage of AGTC equipment by staff per client request OR by client with staff support $13.38/h;
Bioanalyzer chip (standard, 1-11 samples) $39.16
Bioanalyzer chip (high sensitivity, 1-11 samples) $71.28
DNA quantification with PicoGreen (1-24 samples) $29.97
DNA quantification with Qubit (per sample) $1.95
RNA quantification with Qubit (per sample) $2.46
Robotic replication of 96- or 384-well plates (2 copies of 8 x 96-/384-well plates) $126.83/ 16 plates
Load and run TLDA cards on ABI HT7900 RT-PCR machine $65.35/card
Client use of Hydroshear $22.03/h
Client use of a single HiGro incubator pod (6 deep well plates per pod) $18.92/h 
Client use of thermal cycler $7.29/h 
Client use of non-robotic equipment $7.25/h 
Client use of GenoGrinder (grinding of one pair of plates constitutes a run) $22.68/run 

 


MiSeq Services
Schedule 38 - MiSeq run (2X250 flowcell) $1,317.74
Schedule 39A - Nextera Library Prep $321.09
Schedule 39B - Nextera XT Library Prep $325.41
Schedule 40A - TruSeq Library Prep $316.76
Schedule 40B - TruSeq PCR free DNA Prep $287.76
Schedule 41A - TruSeq RNA Prep $343.78
Schedule 41B - Alternative RNA Library Prep $417.05
Schedule 44 - QPCR for NGS Libraries $27.38

 


Bioinformatic Analysis 

Services are billed at $100/h cpu time