UK-AGTC Rates 

Sanger Sequencing
Run-only: client-prepared, client-purified sequencing reactions (1-16 samples) $3.38/sample
Run-only:  client-prepared, client-purified sequencing reactions (17-96 samples) $54.83 (flat rate)
React & run: AGTC performs sequencing reactions, purification and run (1-19 samples) $8.11/sample
React & run: AGTC performs sequencing reactions, purification and run (20-60 samples) $154.40 *
React & run: AGTC performs sequencing reactions, purification and run (61-94 samples) $194.43 *
Sequence reaction cleanup, ethanol precipitation (1 – 3 samples)  $0.81/sample
Sequence reaction cleanup, ethanol precipitation (4 – 96 samples) $2.70 (flat rate)
Sequence reaction cleanup, Life Technologies BigDye Xterminator (removes dye front) $3.06/sample
PCR product purification: magnetic bead prep $1.21/sample
PCR product purification: enzymatic cleanup (per sample) $2.18/sample
Plasmid DNA purification from bacteria (96-well plate) $172.13/plate
Fosmid/cosmid/BAC purification from bacteria (96-well plate) $229.14/plate

* Please note primer cost: One primer is included in the price. Each additional primer on a plate will be billed at $6.22/primer

 


Ion TorrentTM
Shotgun library preparation $366.31
Template preparation for one 314 or 316 chip $284.86
Sequencing of one 314 chip $318.69 
Sequencing of one 316 chip $572.93

 


454 Sequencing
Shotgun library preparation $810.87
GS Titanium XL run - standard or extended read lengths (Note: user can request ½, ¼ or 1/8, etc. plate to reduce costs.  Charge will be pro-rated accordingly) $4109.95/plate
Large volume emulsion PCR (prorated if run uses less than a whole plate) $1705.85/plate
cDNA DNA library construction with directional adaptors $1978.81
Paired end library 3 kb $1744.13
Paired end library 8 kb $1830.36

 


Fragment Analysis
Fragment Analysis on the ABI 3730 (client performs reactions) $58.61/ 96‑well plate

 


Other AGTC Services
Programming of robot by AGTC staff $202.91/h
Usage of AGTC equipment by staff per client request OR by client with staff support $13.75
Bioanalyzer chip (standard, 1-11 samples) $39.75
Bioanalyzer chip (high sensitivity, 1-11 samples) $76.87
DNA quantification with PicoGreen (1-24 samples) $30.66
DNA quantification with Qubit (per sample) $2.01
RNA quantification with Qubit (per sample) $2.63
Load and run TLDA cards on ABI HT7900 RT-PCR machine $66.06/card
Client use of Hydroshear $19.40/use
Client use of a single HiGro incubator pod (6 deep well plates per pod) $19.01/h 
Client use of thermal cycler $5.43/h 
Client use of GenoGrinder (grinding of one pair of plates constitutes a run) $14.93/run 

 


MiSeq Services
MiSeq run (2X150 cycle flowcell) $1237.53
MiSeq run (2X250 cycle flowcell) $1,317.61
MiSeq run (2X300 cycle flowcell) $1697.19
MiSeq run (Nano 2X250 flowcell) $940.20
Nextera Library Prep $323.24
Nextera XT Library Prep $324.47
TruSeq Library Prep $363.31
TruSeq PCR free DNA Prep $286.94
TruSeq RNA Prep $372.71
QPCR for NGS Libraries $37.83

 


Bioinformatic Analysis 

Services are billed at $99.74/h cpu time    

CPU time associated with bioinformatic consulting (Estimate will be provided before work begins) $9.99/ unit