Room 430, Sanders-Brown Center on Aging
800 South Limestone Street
Lexington, KY 40536-0230
Anatomy and Neurobiology, Neurology and Sanders-Brown Center on Aging
The role of the extracellular matrix in stroke
Front row Left to Right: Justin F. Fraser, MD; Gregory Bix, MD, PhD; Jill Roberts, PhD
Second Row left to right: Stephen Grupke, MD; Amy Gorman, MS; Michael Kahle
My lab is interested in the role of extracellular matrix in stroke, the third leading cause of mortality in the United States. Vascular matrix components are often very sensitive to proteolytic processing/degradation in models of focal cerebral ischemia. Often such processing will generate biologically active matrix fragments. One focus of our lab is to determine how select matrix fragments affect the neurovascular unit, the functional multicellular unit that is perturbed in stroke. Another focus of our lab is to determine what role these matrix fragments could play in neurodevelopment involving processes that are often recapitulated in response to CNS injury.
Kahle MP, Lee B, Pourmohamad T, Cunningham A, Su H, Kim H, Chen Y, McCulloch CE, Barbaro NM, Lawton MT, Young WL, Bix GJ (2012) Perlecan domain V is upregulated in human brain arteriovenous malformation and could mediate the vascular endothelial growth factor effect in lesional tissue. Neuroreport 23:627-630.
Dirisala VR, Jeevan A, Bix G, Yoshimura T, McMurray DN (2012) Molecular cloning and expression of the IL-10 gene from guinea pigs. Gene 498:120-127.
Saini MG, Bix GJ (2012) Oxygen-glucose deprivation (OGD) and interleukin-1 (IL-1) differentially modulate cathepsin B/L mediated generation of neuroprotective perlecan LG3 by neurons. Brain Res 1438:65-74.
Wright S, Parham C, Lee B, Clarke D, Auckland L, Johnston J, Lawrence AL, Dickeson SK, Santoro SA, Griswold-Prenner I, Bix G (2012) Perlecan domain V inhibits alpha2 integrin-mediated amyloid-beta neurotoxicity. Neurobiol Aging 33:1379-1388.
Saini MG, Pinteaux E, Lee B, Bix GJ (2011) Oxygen-glucose deprivation and interleukin-1alpha trigger the release of perlecan LG3 by cells of neurovascular unit. J Neurochem 119:760-771.
Al-Ahmad AJ, Lee B, Saini M, Bix GJ (2011) Perlecan domain V modulates astrogliosis In vitro and after focal cerebral ischemia through multiple receptors and increased nerve growth factor release. Glia 59:1822-1840.
Lee B, Clarke D, Al Ahmad A, Kahle M, Parham C, Auckland L, Shaw C, Fidanboylu M, Orr AW, Ogunshola O, Fertala A, Thomas SA, Bix GJ (2011) Perlecan domain V is neuroprotective and proangiogenic following ischemic stroke in rodents. J Clin Invest 121:3005-3023.
Al Ahmad A, Lee B, Stack J, Parham C, Campbell J, Clarke D, Fertala A, Bix GJ (2010) Endostatin binds nerve growth factor and thereby inhibits neurite outgrowth and neuronal migration in-vitro. Brain Res 1360:28-39.