
ATP Synthesis, Chemiosmotic Hypothesis, and H+ Transport
Outline
1)Cyclic vs non-cyclic e- transport a)advantages b)disadvantages 2)H+-driven ATP synthesis a)stoichiometry b)energetics c)electrochemical potential CF0/CF1 ATP Synthase 1)Structure and Components 2)Characteristics 3)Crystal structure 4)Revolving wheel model 5)Ledger sheets a)H2O oxidation requirements b)CO2 fixation requirements I)C3 II)C4 c)ATP/NADPH
Expressed in molar terms, an Einstein is the amount of energy in
Avogadro's number of photons:
Light driven ATP synthesis, photophosphorylation, is chemiosmotic.
During photosynthesis protons accumulate on the lumen side of thylakoid
membranes setting up a proton gradient. This establishes an
electrochemical gradient, or proton-motive force, across the thylakoid
membranes providing the energy for ATP synthesis as protons are
vectorially transported to the stroma through the ATP synthase
complexes. At least 3 H+ must be translocated for every
ATP synthesized. The 1st direct evidence for the chemiosmotic
hypothesis was obtained by Jagendorf and Uribe in elegantly simple
experiments with isolated
chloroplasts.
This is animated in the third figure on Anthonie Muller's page.

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| All materials © 1998, 1999, 2000, Dr. David Hildebrand or Dr. Bob Houtz, unless otherwise noted. | |||||||
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