QUESTIONS ABOUT PROTEOLYSIS & HORMONES

INSTRUCTORS

PLS 623

Time: 11:00 - 11:50 a.m., MWF
Place: Room A6, ASCN Bldg.

AIMS: This course aims to provide a training ground for the development of presentation and critical reading skills. Students will present key scientific papers selected by the instructors from the Hormone Signaling and the Ion and Solute Transport research fields. Learning to critically read, evaluate and present a research paper is an essential skill for a successful career in science. In addition, students will become familiar with the research seminar format and will obtain detailed insight into the strategies and technologies used to successfully complete Plant Physiology research projects.

FORMAT: The plant hormone signaling section will start with a short introduction on targeted proteolysis, a gene expression control mechanism that is especially important for plant signal transduction pathways. This introduction will follow a classical format with lectures provided by the instructor and ending with an exam. Subsequently, the course will proceed with student presentations according to a journal club format. There will be one presentation per session. Students will be expected to prepare a Power Point presentation of approximately 25 minutes that should be structured as follows:
1. Introduction (approx. 5 min.): Provide essential background information to allow the audience to understand and appreciate the research. Why is this important? What is the hypothesis that is being tested?
2. Results (approx 15 min.): Present the essential data (tables and figures) and the methodology used. Provide a critical analysis of the results. Do the data support the conclusions? Are there alternative explanations?
3. Implications and Future Directions (approx 5 min.): Evaluate the novelty of the conclusions and to what extent they have changed the respective research area. Describe some of the logical next research steps.
The time remaining will be used to provide feedback about the presentation, for group discussion and for a quiz that will include 10 true or false questions about the paper that was discussed.

GRADING: Grades will be based on the quality of the presentations, and on the exam and quizzes.

Topic I: Plant Hormone Signaling - Dr. J. Smalle (jsmalle@uky.edu)
Module I: Introduction to targeted protein degradation
Jan 11
Download Lectures 1 & 2.ppt
Jan 13  
Jan 18 Download Lecture 3.ppt
Jan 20 Download Lecture 4.ppt
Jan 23 Exam (12% of grade)
Module II: Student presentations
Jan 25 Auxin
Jan 27 Auxin
Jan 30 Auxin
Feb 1 Auxin
Feb 3 Auxin
Feb 6 Auxin
Feb 8 Auxin
Feb 10 Ethylene
Feb 13 Ethylene
Feb 15 Ethylene
Feb 17 Ethylene
Feb 20 Ethylene
Feb 22 Ethylene
Feb 24 Ethylene
Feb 27 Cytokinin
Feb 29 Cytokinin
March 2 Cytokinin
March 5 Cytokinin
March 7 Cytokinin
March 9 Cytokinin
March 19 Cytokinin
March 21 Gibberellin
March 23 Gibberellin
March 26 Gibberellin
March 28 Gibberellin
March 30 Gibberellin
April 2 Gibberellin
April 4 Gibberellin
Presentations and quizzes: 65% of grade
Topic II: Ion and solute transport - Dr. G. Wagner (gwagner@uky.edu)
Module III: Membrane and long distance transport
April 6 Download Lecture
April 9 Download Lecture
April 11 Download Lecture
April 13 Download Lecture
Module IV: Student presentations
April 16 Solutes
April 18 Energetics
April 20 Energetics
April 23 Transport Proteins and Complexes
April 25 Transport Proteins and Complexes
April 27 Long Distance Transport
Presentations and quizzes: 23% of grade