We are in the process of developing a manual describing experimental and equipment procedures. The protocols are secured in a page open only to members of the Protein lab and may only be accessed by logging into Sue's closed Portal. Click the link below to be taken to the corresponding Section containing documents. Below the sections is list containing the procedures you will find in the secure location. For login access, please contact Sue.

Protein Isolation

• Pork MPI

Equipment Procedures

• Accumet AB15 pH Meter
• Bohlin Rheometer
• C10 Shaker
• Differential Scanning Calorimetry (DSC)
• Fluoromax-3 Fluorometer
• Gradient Gel Electrophoresis
• Hoefer Mighty Small Gel (SDS-PAGE Old Apparatus)
• Instron
• Isotemp 3013 Waterbath
• SDS-PAGE (Biorad Small Gel Apparatus)
• UV 1601 Spec (Room 416)

Experiment Procedures

• ATPase Activity (Calcium and Potassium)
• Babcock Fat Determination
• Biorad Protein Assay
• Biuret Protein Concentration Determination
• Carbonyl Assay
• SDS-PAGE Molecular Weight Standard preparation (Biorad)
• SDS-PAGE Recipes (different percentages of gels)
• Sulfhydryl Assay
• Thiobarbituric Acid Reactive Substances (TBAR)