Plant Physiology I
Cell components and mutants useful in elucidating plant developmental events:
Arrangement is by section and lecture.
Section I: Introduction:
Lecture I: Introduction to Plant Development:
Lecture II: Introduction to Tissues and Cell Types:
Lecture III: Cell Division, Expansion, and Communication:
root meristemless mutants (rml1 and rml2): rml1 is allelic (phenotype determined by different alleles of the same gene i.e. the same gene has been mutated at different positions) with the cadmium sensitive2 mutant. The radicle does not elongate more than 2 mm due to a lack of cell division. Caused by a deficiency in gluathione due to an aberration in an enzyme of the biosynthetic pathway.
korrigan (kor): kor mutants are defective in a putatively membrane-bound cellulase possibly associated with cellulose biosynthesis. The mutants are extremely dwarfed with many cells that fail to expand due to a breakdown in the correct assembly of the primary cell wall.
knolle (kn1): "Surface of seedling appears rough due to lack of a well-formed epidermal layer (radial pattern deffect which affects only development of the pattern); large cells enclose lumps of vascular tissue; the outer layer of epidermal precursor cells of knolle globular embryos cannot be distinquished from inner cells; large, irregularly spaced cells are present at this stage; maintained as heterozygote (seedling lethal/embryo defective)." (Arabidopsis Information Management System, 1999).
Five main components of plant cell walls;
1) cellulose; 2) hemicellulose; 3) pectin; 4) protein; 5) water.
Ions such as calcium make up a sixth component but in truly negligible amounts relative to the first 5.
Section II: Embryo and Seed Development:
Lecture IV and V: Embryogenesis:
Lecture VI: Endosperm Development:
Lecture VII: Apomixis and Imprinting:
Lecture VIII: Seed and Fruit Development:
Diageotripica (dgt): Vigorous growth in greenhouse but prostrate; concave cotelydons. Roots grow horizontally.
Lecture IX and X: Germination and Dormancy:
gib-1: gibberellin responsive dwarf; small, dark green leaves; requires gibberellin for germination and seed set.
Section III: Vegetative Development:
Lectures XI: Trichomes, Stomata, Root Hairs:
Reduced Trichome Number (RTN): This gene is probably responsible for determining when leaf cells are competent for producing trichomes during their development. It is active for a longer period in the first leaf of the arabidopsis ecotype columbia (Col) than it is in the Landsberg erecta ecotype (Ler), resulting in the "hairy" phenotype of Col relative to Ler.
glabra1 (gl1): Trichomless mutant of arabidopsis. Deficient in trichome development.
transparent testa glabra (ttg): Trichomless mutant of arabidopsis. Deficient in trichome development, anthocyanin production, and seed coat mucilage. "Yellow seeds due to absence of brown pigment in seed coat (testa); anthocyanins absent in leaves, stems and all tissues; absence of the ruthenium red-staining seed mucilage, abnormal appearance of cells of dry seed coat in scanning electron microscope; no trichomes on leaf surfaces or stem base." (Arabidopsis Information Management System, 1999).
tryptychon (try): This mutant has trichomes that occur in clusters rather than the normal dispersed phenotype.
four lips (flp): This mutation typically has two stoma positioned side by side resulting in four guard cells in contact.
too many mouths (tmm): This mutation also violates the rule that no two stomata should form so as to be in contact. In this mutation many stomata form in a cluster.
R-558: This mutation can cause increased stomatal occurrence per unit leaf area and can also cause stomatal clustering.
Vascular tissue differentiation:
Companion cell proton pump, AHA3
Companion cell proton-sucrose symporter, SUC2
Sieve element proton-sucrose symporter, SUT1
sieve element reticulum, SER
Tracheary element differentiation-related gene, TED
TED2: an NADPH-oxidoreductase
avirulence gene, Avr9
resistance gene, Cf-9
oligosaccharyltransferase-2, Ost-2
Lecture XVI: Root Development:
fass (fas): Produces excessive numbers of cells in the root meristem of arabidopsis. "Fasciated plant; narrow, dentated leaves; altered flower development and phyllotaxy; somewhat reduced fertility." (Arabidopsis Information Management System, 1999).
tonneau (ton): Produces excessive numbers of cells in the root meristem of arabidopsis.
shortroot (shr): lacks the endodermal tissue layer.
scarecrow (scr): lacks one of the two ground tissue layers (its initials have the characteristics of both endodermal and cortical cells). "greatly reduced root length; aerial portion of plant appears similar to wild type; single layer only of cells occurs between the epidermis and pericycle (lacks radial organization in cortex and endodermis); occasionally, more than the expected 8 cells occur in the mutant layer; extra cells also occasionally occur in epidermis; apparently involve a defect of the division of the cortex/endodermal initial cells; a similar defective division also occurs in the embryonic ground tissue; member of a novel family having similarity to DNA binding proteins containing basic-leucine zipper regions; scr is expressed in cortex/endodermal initial cells and in the endodermal cell lineage." (Arabidopsis Information Management System, 1999).
gollum (glm): alters the organization of vascular tissue and pericycle.
wooden leg (wol): alters the vascular tissue _______.
Lecture XIV: SAM and Initiation of Organs:
Lecture XV: Juvenile and Adult Vegetative Development:
Section IV: Reproductive development:
Lecture XVII and XVIII: Transition to Flowering:
leafy (lfy) "strong allele; early-arising flowers completely transformed into inflorescence shoots, late-arising flowers partially transformed; petals and stamens absent; sterile; more severe phenotype under short days and lower temperature; maintained as heterozygote." (Arabidopsis Information Management System, 1999).
terminal flower (tfl): "strong allele, early flowering, reduced plant height and increased number of rosette inflorescences; determinate inflorescences; terminal region of primary inflorescence may consist of a single flower or of 2-3 clustered flowers separated by short internodes, in which organs of the 3 outer whorls are missing or are mosaic; secondary inflorescence apices are determinate and usually converted into a single normal flower; 2-5 normal flowers/inflorescence; photoperiod affects phenotype." (Arabidopsis Information Management System, 1999).
Lecture XIX and XX: Flower Development:
Lecture XXI and XXII: Gamete Development:
Lecture XXIII: Pollination:
Mutants in pollen release:
(msH): arabidopsis mutant that fails to release pollen upon maturation.
(ps): tomato mutant that fails to release pollen upon maturation.
Mutants in pollen formation:
quartet mutants: qrt1-1: "Outer walls of the four meiotic products of a pollen mother cell are fused and the pollen grains fail to separate, forming pollen tetrads; pollen viable and fertile, and fertilization with a single tetrad usually yields four seeds." (Arabidopsis Information Management System, 1999).
qrt2-1: "Outer walls of the four meiotic products of a pollen mother cell are fused and the pollen grains fail to separate, forming pollen tetrads; pollen viable and fertile, and fertilization with a single tetrad usually yields four seeds." (Arabidopsis Information Management System, 1999).
Mutants in pollen function:
whp:
c2:
Lecture XXIV: Self Incompatability: