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Tracy Bonilla (Undergraduate Researcher)
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I spent the summer of 2005 in the Downie lab and in the University of Kentucky Advanced Genomics Technology Center as a Kentucky Young Scientist Summer (KYSS) Intern. I purified, sheared, polished, and sub-cloned two BACs (Bacterial Artificial Chromosomes) containing fragments of tomato chromosome 1. I helped plate the sub-cloned libraries and used a robot to select E. coli housing plasmids that contained tomato genomic DNA. I then used a second robot to prepare sequencing grade plasmid DNA from randomly selected colonies and to set up cycle sequencing reactions. I also readied the sequencing reactions once they were completed, for submission to the sequencers. We have over 190 000 contiguous bases of tomato chromosome 1 sequenced from my efforts and have used this information to develop 3 CAPS (Cleaved Amplified Polymorphic Sequence) markers and a SSR (Simple Sequence Repeat [microsatellite]) marker between L. esculentum and L. pimpinellifolium using this information. Due to the information I generated, we are one step closer to discovering the identity of the BROWNSEED1 gene.
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