In 2011, the CMB received IRB approval to collect human vastus lateralis muscle samples from healthy volunteers. In addition to providing 200-300 mg of muscle tissue, volunteers undergo body composition evaluation, a fasting blood draw, strength testing, and complete various surveys on activity level and quality of life, coordinated between the Human Performance Lab and Center for Clinical and Translational Sciences. To date, over 100 samples from both males and females ranging in age from 18-82 years old have been acquired. Currently the Center for Muscle Biology will provide frozen muscle sections from these tissues for research. Frozen sections from muscle biopsies are cut at 7μm, in triplicate with a maximum of 4 subjects per slide. Sections are mounted on Superfrost Plus charged slides (Fisher, 12-550-15). Immunohistochemistry, histology, and imaging of common muscle characteristics are also available upon request. Please visit our Muscle Immunohistochemistry and Molecular Imaging Core (MIMIC) for more details.
Human Vastus Lateralis RNA
RNA has been isolated from muscle of a subset of healthy volunteers. Total RNA was extracted using homogenization in QIAGEN QIAzol Lysis reagent (Cat No. 79306) followed by column-based RNA washing with the QIAGEN miRNeasy Mini Kit (Cat No. 217004), according to manufacturer protocol. RNA quality was assessed using the Agilent 2100 Bioanalyzer. Total RNA costs $76/per 1 µg.
Human Primary Myoblasts
The CMB maintains a repository of human primary myoblasts isolated from vastus lateralis biopsies. Upon request, detailed medical history, exercise physiology, and metabolic data are available for some donors. Cells were isolated using collagenase/dispase digest followed by magnetic bead sorting for CD56/NCAM+ cells. Please see Varma et al. for detailed methods of human primary myoblast isolation1.
A vial costs $300 for internal University of Kentucky researchers and $399 for external academic researchers (1 vial= approximately 250,000 cells). To make a request, please fill out the Service Request Form and refer to our Rates page for more details. If you have a special request, please contact us.
1. Varma V, Yao-Borengasser A, Rasouli N, Nolen GT, Phanavanh B, Starks T, Gurley C, Simpson P, McGehee RE, Jr., Kern PA, and Peterson CA. Muscle inflammatory response and insulin resistance: synergistic interaction between macrophages and fatty acids leads to impaired insulin action. American Journal of Physiology Endocrinology and Metabolism. 296: E1300-1310, 2009.