MyoVision: automatic image quantification platform


1. About MyoVision
2. Core Features
3. Download
4. Documentation


About MyoVision

​Immunfluorescence microscopy is a valuable tool in the study of skeletal muscle biology. High content imaging allows for the simultaneous measurement of muscle cellular morphology and biomarker quantification. The increasing speed of automated microscopy can result in enormous bioimage datasets. Technology for generating high content images has outpaced the current manual and semi-automatic methods of image analyses. For routine morphometry such as cell size, fiber type, and myonuclear counting, the sheer volume of bioimage data demands full automation.

MyoVision is made freely available to remove the heavy demand on human attention during image analysis. It incorporates and expands upon multiple algorithms specifically developed for skeletal muscle morphormetry to achieve high degrees of accuracy and consistency. Currently, MyoVision Basic v1.0 only includes basic analytical features, supports limited user interactivity, and is restricted to Microsoft Windows. However, MyoVision will act as the foundation of a power analytical platform that will incorporate more advanced features, expand biomarker multiplexing capabilities, and support all operating systems.

Wen Y, Murach KA, Vechetti IJ, Jr., Fry CS, Vickery CD, Peterson CA, McCarthy JJ, and Campbell KS. MyoVision: Software for Automated High-Content Analysis of Skeletal Muscle Immunohistochemistry. J Appl Physiol. jap 00762 02017, 2017.


Core Features

Cell Detection and Counting

  • ​MyoVision uses a laminin or dystrophin immunofluorescence of as a reference image to detect and count myofibers on cross section.

Cell Size Measurement

  • Once myofiber cytoplasmic regions are detected and outlined, area and minimum Feret diameters are calculated for each cell.

Fiber Type Classification

  • Using the cytoplasmic outlines generated from the reference image as a mask, additional channels of myosin heavy chain immunofluorescence is quantified and used to classify fiber types.

Myonuclear Counting

  • If the reference image is dystrophin immunofluorescence, the cytoplasmic mask can be applied to the DAPI channel to quantify myonuclear number.



If you would like to download the MyoVision Basic Installer Package, please fill out the webform at the following link: MyoVision Basic Downloads Form

System Requirements:
     Operating System: Windows 10
     CPU: Intel i7-6700K
     RAM: 16 GB (More if better)

Please note that MyoVision Basic is intended for the analysis of relatively small images one at a time in order to see the intermediate steps. Also, typical laptops and desktops may be too slow for large cross sections and high volume analyses. The Center for Muscle Biology provides analysis services using a dedicated high performance server (32 CPU cores with 250 GB of RAM). For more information, please see MyoVision Pro



Quick-Start Guide 

Fiber Type Demonstration Video