Seed collection: Ohio buckeye fruits are capsules. They can be collected in late summer after they turn a leathery tan color and begin to split open exposing the three large black seeds.
Seeds are removed by peeling the capsule apart. Seeds resemble edible chestnuts, but Ohio buckeye fruits are not edible and can be toxic.
Seeds of Ohio buckeye should not be allowed to dry out. They can be stored for short periods (1 year) in air tight containers in the refrigerator if not allowed to dry out.
Seed dormancy: Ohio buckeye has physiological dormancy.
Seed germination: Stratify seeds using moist chilling for 120 days to satisfy physiological dormancy. Following stratification, sow seeds in a nursery container to produce a seedling or sow them in a plastic container in the classroom to observe germination.