BIO 510 Recombinant DNA Techniques Laboratory

Instructors: Professors G. Jones & J. Rawlss is a laboratory course designed to supplement instruction in the Genetics (BIO 304and Cell Biology (BIO 315) courses for biological sciences majors at the University of Kentucky. Additional information may be had at the following links:

BIO 510 Course Syllabus and Schedule of Experiments (Fall 2002)

A lab coat and lab notebook are required. Guidelines for the lab notebook are available here.

The course is divided into two parts. The links below will connect you to the relevant lab protocols:

ANALYSIS OF AN ENHANCER TRAP LINE (Dr. Rawls, Coordinator)

• Genomic DNA Preparation (August 28-September 9)
• Restriction and Southern Blotting (September 6-11)
• Probe Labelling and Blot Processing (September 13-23)
• Inverse PCR (September 13-25)
• Cloning a PCR Fragment into pBS (September 25-October 2)
• DNA Sequencing (October 7-14)
• lacZ Staining of Tissues (October 14-16)
• Bioinformatics: Using BLASTN, FlyBase & BDGP Informatics (October 16-25)
  

ANALYSIS OF A HORMONE RESPONSIVE PROMOTER (Dr. Jones, Coordinator)s ilaboratory course designed to supplement instruction in nal information ay be had at the following links:

• Dr. Jones Lectures
• Dr. Jones Lab Sessions
• Dr. Jones Pop Quizzes