LEA Phage Display Hits
In-frame hits from phage display pans with SMP1 (AT3G12960) from Arabidopsis seed library (SL) 1 and SL2 at 25°C and 41°C. Hits: the total number of sequences encountered encoding this protein for this library, whether it was identical or not. Independent clones: those encoding the same protein but from different pans of the same library or different lengths cDNA.
| In-frame hits of SMP1 at 25°C using SL1 after 4 rounds of panning | ||||
|---|---|---|---|---|
| Hits | Locus Identifier | Protein Identity | Independent clones | Authority/Subcellular Residence |
| 6 | AT1G15280 | CASC3/Barentsz eIF4AIII binding | 4 | Predictors: SubLoc - nucleus; WoLFPSORT – nucleus |
| 2 | AT1G30610 | EMB88, EMB2279 pentatricopeptide (PPR) repeat-containing protein | 2 | Predictors: iPSORT - plastid; MitoPred - mitochondrion; MultiLoc - plastid; PeroxP - peroxisome; Predotar - plastid; SubLoc - nucleus; TargetP – plastid; WoLFPSORT – nucleus |
| 1 | AT1G58210 | EMB1674 | kinase interacting family protein | 1 | Predictors: iPSORT - mitochondrion; MitoPred - mitochondrion; MultiLoc - plastid; Predotar - mitochondrion; SubLoc - nucleus; TargetP - plastid; WoLFPSORT - nucleus |
| 1 | AT3G55170 | Ribosomal L29 family protein | 1 | SUBA MS/MS: cytosol - 17934214; plasma membrane – 19334764 Predictors: LOCtree - plastid; MitoPred - mitochondrion; SubLoc - nucleus; WoLFPSORT - nucleus |
| In-frame hits of SMP1 at 41°C using SL1 after 4 rounds of panning | ||||
| Hits | Locus Identifier | Protein Identity | Independent clones | Authority/Subcellular Residence |
| 2 | AT1G15290 | Tetratricopeptide repeat (TPR)-like superfamily | 1 | SUBA MS/MS: cytosol – 21166475 Predictors: SubLoc - nucleus; WoLFPSORT - nucleus |
| 1 | AT1G16610 | SR45, RNPS1 | arginine/serine-rich 45 | | 1 | Annotators: BAR Cell eFP Browser - Mitochondria, nucleus plastid |
| 1 | AT1G30610 | EMB88, EMB2279 pentatricopeptide (PPR) repeat-containing protein | 1 | Predictors: iPSORT - plastid; MitoPred - mitochondrion; MultiLoc - plastid; PeroxP - peroxisome; Predotar - plastid; SubLoc - nucleus; TargetP - plastid; WoLFPSORT - nucleus |
| 1 | AT1G55310 | SR33, SCL33, At-SCL33 | SC35-like splicing factor | 1 | Annotators: TAIR - interchromatin granule, nuclear speck, nucleolus, plasma membrane |
| 1 | AT2G31410 | unknown protein | 1 | Predictors: MitoPred - mitochondrion; SubLoc - nucleus; WoLFPSORT – nucleus |
| 3 | AT2G36640 | ATECP63, ECP63 embryonic cell protein 63 | 2 | Predictors: LOCtree - cytosol; SubLoc – nucleus; WoLFPSORT - peroxisome |
| 1 | AT5G46430 | Ribosomal protein L32e | 1 | SUBA MS/MS: cytosol - 17934214; cytosol - 21166475; plasma membrane - 19334764; plasma membrane - 15574830 Predictors: iPSORT - mitochondrion; LOCtree - mitochondrion; MitoPred - mitochondrion; Mitoprot 2 - mitochondrion; MultiLoc - plastid; Predotar - mitochondrion; SubLoc - mitochondrion; TargetP - mitochondrion; WoLFPSORT - cytosol |
| In-frame hits of SMP1 at 25°C using SL2 after 4 rounds of panning | ||||
| Hits | Locus Identifier | Protein Identity | Independent clones | Authority/Subcellular Residence |
| 2 | AT1G11630 | Pentatricopeptide repeat-containing protein | 2 | SUBA MS/MS: mitochondrion : 14671022 Predictors: iPSORT - mitochondrion; LOCtree - mitochondrion; MitoPred - mitochondrion; MultiLoc - plastid; Predotar - mitochondrion; SubLoc - nucleus; TargetP - mitochondrion; WoLFPSORT – plastid |
| 3 | AT1G65090 | unknown protein | 3 | Annotators: BAR Cell eFP Browser - cytosol, mitochondria |
| In-frame hits of SMP1 at 41°C using SL2 after 4 rounds of panning | ||||
| Hits | Locus Identifier | Protein Identity | Independent clones | Authority/Subcellular Residence |
| 3 | AT1G15280.2 | CASC3/Barentsz eIF4AIII binding | 3 | Predictors: SubLoc - nucleus; WoLFPSORT - nucleus |
| 3 | AT1G15290.1 | Tetratricopeptide repeat (TPR)-like superfamily | 1 | SUBA MS/MS: cytosol :-21166475 Predictors: SubLoc - nucleus; WoLFPSORT - nucleus |
| 1 | AT1G54870.1 | NAD(P)-binding Rossmann-fold superfamily protein | 1 | Predictors: MultiLoc - mitochondrion; SubLoc - cytosol; WoLFPSORT - plastid |
| 1 | AT3G01690.1 | alpha/beta-Hydrolases superfamily protein | 1 | Predictors: iPSORT - mitochondrion; SubLoc - nucleus; TargetP - plastid; WoLFPSORT – cytosol |
| 1 | AT3G58680.1 | MBF1B, ATMBF1B | multiprotein bridging factor 1B | 1 | GFP: cytosol - 15610358; nucleus – 15610358 Annotators: AmiGO - nucleus Predictors: LOCtree - cytosol; MitoPred - mitochondrion; Predotar - plastid; SubLoc - nucleus; WoLFPSORT - nucleus |
| 1 | AT4G12400.2 | Hop3 | stress-inducible protein, putative | 1 | No information |
| 1 | AT5G52240.1 | MSBP1, ATMP1, AtMAPR5 membrane steroid binding protein1 | 1 | SUBA MS/MS: endoplasmic reticulum - 16618929; plasma membrane - 17317660; plasma membrane - 19334764; plasma membrane - 17644812 Annotators: AmiGO - plasma membrane; Predictors: SubLoc - cytosol; TargetP - extracellular; WoLFPSORT - endoplasmic reticulum |
