Title | Role of oil vehicle on hepatic cell proliferation in PCB-treated rats. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Bunaciu RPetruta, Tharappel JC, Lehmler H-J, Lee EY, Robertson LW, Bruckner G, Spear BT, Glauert HP |
Journal | J Environ Pathol Toxicol Oncol |
Volume | 30 |
Issue | 4 |
Pagination | 273-82 |
Date Published | 2011 |
ISSN | 2162-6537 |
Keywords | Animals, Aryl Hydrocarbon Hydroxylases, Cell Proliferation, Corn Oil, Cytochrome P-450 CYP2B1, Glycine, Liver, Male, Oils, Olive Oil, Pharmaceutical Vehicles, Plant Oils, Polychlorinated Biphenyls, Rats, Rats, Sprague-Dawley, Steroid Hydroxylases, Triglycerides |
Abstract | We report the role of dietary glycine and the type of oil used as a vehicle in the hepatotoxicity of control rats and rats treated with 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153). In our first experiment, glycine or valine (as control) was fed in an unrefined diet at 5% for the entire study duration (5 days) to inhibit Kupffer cell activity. PCB-153 (100 or 300 μmol/kg) dissolved in medium chain triglyceride (MCT) oil, was injected intraperitoneally 2 days before euthanasia; the peroxisome proliferator Wy-14,643 was included as a positive control. MCT oil decreased cell proliferation by approximately 50%. PCB-153 slightly increased hepatic cell proliferation, but dietary glycine did not reduce cell proliferation. Because of the inhibition of cell proliferation in rats receiving MCT oil compared with rats receiving no injection, we hypothesized that MCT oil may have been inhibiting the hepatocyte proliferation in PCB-153-treated rats. We therefore performed another experiment using 3 types of oil as a vehicle for PCB-153: MCT oil, corn oil, and olive oil. Rats were injected with PCB-153 (300 μmol/kg) or one of the vehicles, again 2 days before euthanasia. MCT oil again decreased the hepatocyte proliferation by approximately 50%. In rats receiving PCB-153, hepatocyte proliferation was slightly higher than their respective vehicle controls for corn oil and olive oil but not for MCT oil. These studies show that the oil vehicle is important in cell proliferation after PCB exposure, with MCT oil appearing to be protective. |
DOI | 10.1615/jenvironpatholtoxicoloncol.v30.i4.10 |
Alternate Journal | J. Environ. Pathol. Toxicol. Oncol. |
PubMed ID | 22181977 |
PubMed Central ID | PMC3522147 |
Grant List | ES013661 / ES / NIEHS NIH HHS / United States P30 ES005605 / ES / NIEHS NIH HHS / United States P42 ES007380 / ES / NIEHS NIH HHS / United States ES07380 / ES / NIEHS NIH HHS / United States P42 ES013661 / ES / NIEHS NIH HHS / United States |