Title | Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Fry CS, Lee JD, Jackson JR, Kirby TJ, Stasko SA, Liu H, Dupont-Versteegden EE, McCarthy JJ, Peterson CA |
Journal | FASEB J |
Volume | 28 |
Issue | 4 |
Pagination | 1654-65 |
Date Published | 2014 Apr |
ISSN | 1530-6860 |
Keywords | Animals, Cell Proliferation, Cells, Cultured, Cellular Microenvironment, Dystrophin, Extracellular Matrix, Fibroblasts, Hypertrophy, Immunohistochemistry, Male, Mice, Inbred C57BL, Mice, Transgenic, Muscle Fibers, Skeletal, Muscle, Skeletal, Myosin Heavy Chains, Oligonucleotide Array Sequence Analysis, PAX7 Transcription Factor, Satellite Cells, Skeletal Muscle, Stress, Mechanical, Tamoxifen, Time Factors, Transcriptome, Weight-Bearing |
Abstract | Our aim in the current study was to determine the necessity of satellite cells for long-term muscle growth and maintenance. We utilized a transgenic Pax7-DTA mouse model, allowing for the conditional depletion of > 90% of satellite cells with tamoxifen treatment. Synergist ablation surgery, where removal of synergist muscles places functional overload on the plantaris, was used to stimulate robust hypertrophy. Following 8 wk of overload, satellite cell-depleted muscle demonstrated an accumulation of extracellular matrix (ECM) and fibroblast expansion that resulted in reduced specific force of the plantaris. Although the early growth response was normal, an attenuation of hypertrophy measured by both muscle wet weight and fiber cross-sectional area occurred in satellite cell-depleted muscle. Isolated primary myogenic progenitor cells (MPCs) negatively regulated fibroblast ECM mRNA expression in vitro, suggesting a novel role for activated satellite cells/MPCs in muscle adaptation. These results provide evidence that satellite cells regulate the muscle environment during growth. |
DOI | 10.1096/fj.13-239426 |
Alternate Journal | FASEB J. |
PubMed ID | 24376025 |
PubMed Central ID | PMC3963024 |
Grant List | UL1 TR000117 / TR / NCATS NIH HHS / United States R21 AG034453 / AG / NIA NIH HHS / United States AG-34453 / AG / NIA NIH HHS / United States AR-60701 / AR / NIAMS NIH HHS / United States R01 AR060701 / AR / NIAMS NIH HHS / United States T32 HL086341 / HL / NHLBI NIH HHS / United States T32-HL-086341 / HL / NHLBI NIH HHS / United States UL1-TR-000117 / TR / NCATS NIH HHS / United States K12 DE023574 / DE / NIDCR NIH HHS / United States |