Title | Myogenic Progenitor Cells Control Extracellular Matrix Production by Fibroblasts during Skeletal Muscle Hypertrophy. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Fry CS, Kirby TJ, Kosmac K, McCarthy JJ, Peterson CA |
Journal | Cell Stem Cell |
Volume | 20 |
Issue | 1 |
Pagination | 56-69 |
Date Published | 2017 01 05 |
ISSN | 1875-9777 |
Keywords | Animals, Carrier Proteins, Cell Differentiation, Cell Nucleus, Cell Survival, Collagen, Down-Regulation, Exosomes, Extracellular Matrix, Fibroblasts, Gene Deletion, Gene Knockdown Techniques, Hypertrophy, Mice, MicroRNAs, Models, Biological, Muscle Development, Muscle Fibers, Skeletal, Muscle, Skeletal, NIH 3T3 Cells, PAX7 Transcription Factor, Ribonuclease III, Satellite Cells, Skeletal Muscle, Stem Cells, Tamoxifen |
Abstract | Satellite cells, the predominant stem cell population in adult skeletal muscle, are activated in response to hypertrophic stimuli and give rise to myogenic progenitor cells (MPCs) within the extracellular matrix (ECM) that surrounds myofibers. This ECM is composed largely of collagens secreted by interstitial fibrogenic cells, which influence satellite cell activity and muscle repair during hypertrophy and aging. Here we show that MPCs interact with interstitial fibrogenic cells to ensure proper ECM deposition and optimal muscle remodeling in response to hypertrophic stimuli. MPC-dependent ECM remodeling during the first week of a growth stimulus is sufficient to ensure long-term myofiber hypertrophy. MPCs secrete exosomes containing miR-206, which represses Rrbp1, a master regulator of collagen biosynthesis, in fibrogenic cells to prevent excessive ECM deposition. These findings provide insights into how skeletal stem and progenitor cells interact with other cell types to actively regulate their extracellular environments for tissue maintenance and adaptation. |
DOI | 10.1016/j.stem.2016.09.010 |
Alternate Journal | Cell Stem Cell |
PubMed ID | 27840022 |
PubMed Central ID | PMC5218963 |
Grant List | UL1 TR000117 / TR / NCATS NIH HHS / United States R21 AG034453 / AG / NIA NIH HHS / United States F32 AR065337 / AR / NIAMS NIH HHS / United States R01 AR060701 / AR / NIAMS NIH HHS / United States P30 AG024832 / AG / NIA NIH HHS / United States UL1 TR001998 / TR / NCATS NIH HHS / United States R01 AG049806 / AG / NIA NIH HHS / United States |