Title | PAX7+ satellite cells in young and older adults following resistance exercise. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Walker DK, Fry CS, Drummond MJ, Dickinson JM, Timmerman KL, Gundermann DM, Jennings K, Volpi E, Rasmussen BB |
Journal | Muscle Nerve |
Volume | 46 |
Issue | 1 |
Pagination | 51-9 |
Date Published | 2012 Jul |
ISSN | 1097-4598 |
Keywords | Adult, Age Factors, Aged, Aging, Exercise, Female, Humans, Male, PAX7 Transcription Factor, Phosphorylation, Quadriceps Muscle, Resistance Training, Satellite Cells, Skeletal Muscle |
Abstract | INTRODUCTION: Resistance exercise (RE) stimulates a muscle protein anabolic response partially through enhanced satellite cell (SC) activity, however, age- and gender-related changes in SC content over a 24-h time course are not known. METHODS: Ten young (27 ± 2 years) men and women and 11 older (70 ± 2 years) men and women performed an acute bout of RE. Myofiber and SC characteristics were determined from muscle biopsies of the vastus lateralis using immunohistochemistry. Immunoblotting was used to determine phosphorylation of cyclin-dependent kinase-2 and protein expression of p27(Kip1) and cyclin D1. RESULTS: Pax7+ SC were significantly increased in young men 24 h following RE. Percent SC were significantly increased in older women at 6 and 24 h following RE. Aging decreased myonuclear domain and increased protein expression of p27(Kip1) . CONCLUSIONS: An acute bout of RE increases SC content in young men at 24 h and older women at 6 and 24 h. |
DOI | 10.1002/mus.23266 |
Alternate Journal | Muscle Nerve |
PubMed ID | 22644638 |
PubMed Central ID | PMC3374905 |
Grant List | T32 HD007539 / HD / NICHD NIH HHS / United States R01AR049877 / AR / NIAMS NIH HHS / United States R01 AR049877-09 / AR / NIAMS NIH HHS / United States UL1 TR000071 / TR / NCATS NIH HHS / United States P30AG024832 / AG / NIA NIH HHS / United States R01 AR049877 / AR / NIAMS NIH HHS / United States UL1-RR029876 / RR / NCRR NIH HHS / United States P30 AG024832 / AG / NIA NIH HHS / United States T32HD07539 / HD / NICHD NIH HHS / United States UL1 RR029876 / RR / NCRR NIH HHS / United States |