The Star Excursion Balance Test (SEBT) is a reliable inexpensive tool used to assess dynamic postural control deficits and efficacy in the prediction of musculoskeletal injuries, but with little previous consideration for performance differences across age and skill levels. The purpose of this study was to examine differences in SEBT scores between high school and collegiate football players. Three-hundred eighteen high school football players and 180 National Collegiate Athletic Association Division I collegiate football players volunteered to participate. Star Excursion Balance Test scores were obtained bilaterally for anterior (ANT), posterolateral (PL), and posteromedial (PM) directions, and for an overall composite (COMP) score. The mean of 3 trials from each leg was normalized to stance leg length and presented as a percentage score. Bilaterally averaged scores were compared between high school and collegiate football players using separate independent t-tests. A multiple linear backward regression determined the amount of variance in SEBT scores explained by age, mass, and height. Compared with collegiate athletes, high school athletes had lower PL (72.8 ± 11.4% vs. 77.1 ± 10.2%; p < 0.001), PM (83.5 ± 10.2% vs. 86.7 ± 10.7%; p = 0.001), and COMP (75.4 ± 8.5% vs. 78.0 ± 7.4%; p = 0.001) scores. Anterior scores did not differ between high school (69.9 ± 7.9%) and collegiate (70.3 ± 7.1%) athletes (p = 0.545). Age, mass, and height were not meaningful contributors to ANT (R = 0.089; p < 0.001), PL (R = 0.032; p < 0.001), PM (R = 0.030; p = 0.002), and COMP (R = 0.048; p < 0.001) variances. Disparity between high school and collegiate athletes should be considered when using the SEBT to identify risk of or deficits related to lower extremity injury in football players.